Reference - Detail
| RRC ID | 36350 |
|---|---|
| Author | Inagaki Y, Matsumoto Y, Sekimizu K. |
| Title | Using silkworms to establish alternative animal models for evaluation of drug-induced tissue injury. |
| Journal | Drug Discov Ther |
| Abstract |
Evaluation of tissue injury induced by chemicals is crucial to drug development. Mice and rats, which are effectively used to analyze drug-induced tissue injury, present problems in terms of cost and ethical issues. Although alternative methods have been developed using in vitro techniques or invertebrates, evaluation of ADME and the size of animals are still issues that need to be addressed. Use of silkworms can resolve these problems. Silkworms have pharmacokinetic characteristics similar to those of mammals. Injection of various hepatotoxic chemicals also leads to elevated alanine aminotransferase (ALT) activity in the hemolymph of silkworms. Furthermore, transparent transgenic silkworms expressing GFP have been produced to facilitate continuous analysis without the need to collect hemolymph. Analyses using this silkworm have indicated that the intensity of GFP fluorescence observed on the body surface of the silkworm decreases in a time- and dose-dependent manner when hepatotoxic chemicals are injected. These results suggest that the silkworms can serve as alternative animal model for evaluation of drug-induced tissue injury. |
| Volume | 10(1) |
| Pages | 40-3 |
| Published | 2016-2-1 |
| DOI | 10.5582/ddt.2016.01023 |
| PMID | 26971554 |
| MeSH | Alanine Transaminase / metabolism Animals Animals, Genetically Modified Biomarkers / metabolism Bombyx / drug effects* Bombyx / embryology Bombyx / genetics Bombyx / metabolism Chemical and Drug Induced Liver Injury / etiology* Chemical and Drug Induced Liver Injury / genetics Chemical and Drug Induced Liver Injury / metabolism Disease Models, Animal Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics Hemolymph / metabolism Humans Insect Proteins / metabolism Larva / drug effects Species Specificity |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | |