RRC ID 3637
Author Totong R, Achilleos A, Nance J.
Title PAR-6 is required for junction formation but not apicobasal polarization in C. elegans embryonic epithelial cells.
Journal Development
Abstract Epithelial cells perform important roles in the formation and function of organs and the genesis of many solid tumors. A distinguishing feature of epithelial cells is their apicobasal polarity and the presence of apical junctions that link cells together. The interacting proteins Par-6 (a PDZ and CRIB domain protein) and aPKC (an atypical protein kinase C) localize apically in fly and mammalian epithelial cells and are important for apicobasal polarity and junction formation. Caenorhabditis elegans PAR-6 and PKC-3/aPKC also localize apically in epithelial cells, but a role for these proteins in polarizing epithelial cells or forming junctions has not been described. Here, we use a targeted protein degradation strategy to remove both maternal and zygotic PAR-6 from C. elegans embryos before epithelial cells are born. We find that PKC-3 does not localize asymmetrically in epithelial cells lacking PAR-6, apical junctions are fragmented, and epithelial cells lose adhesion with one another. Surprisingly, junction proteins still localize apically, indicating that PAR-6 and asymmetric PKC-3 are not needed for epithelial cells to polarize. Thus, whereas the role of PAR-6 in junction formation appears to be widely conserved, PAR-6-independent mechanisms can be used to polarize epithelial cells.
Volume 134(7)
Pages 1259-68
Published 2007-4-1
DOI 10.1242/dev.02833
PII dev.02833
PMID 17314130
MeSH Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Adhesion / physiology* Crosses, Genetic Epithelial Cells / metabolism Epithelial Cells / physiology* Image Processing, Computer-Assisted Intercellular Junctions / physiology* Microscopy, Video Protein Kinase C / metabolism* RNA Interference Transgenes / genetics
IF 5.763
Times Cited 61
C.elegans tm1425