論文 - 詳細
| RRC ID | 36423 |
|---|---|
| 著者 | Chakraborty T, Zhou LY, Chaudhari A, Iguchi T, Nagahama Y. |
| タイトル | Dmy initiates masculinity by altering Gsdf/Sox9a2/Rspo1 expression in medaka (Oryzias latipes). |
| ジャーナル | Sci Rep |
| Abstract |
Despite identification of several sex-determining genes in non-mammalian vertebrates, their detailed molecular cascades of sex determination/differentiation are not known. Here, we used a novel RNAi to characterise the molecular mechanism of Dmy (the sex-determining gene of medaka)-mediated masculinity in XY fish. Dmy knockdown (Dmy-KD) suppressed male pathway (Gsdf, Sox9a2, etc.) and favoured female cascade (Rspo1, etc.) in embryonic XY gonads, resulting in a fertile male-to-female sex-reversal. Gsdf, Sox9a2, and Rspo1 directly interacted with Dmy, and co-injection of Gsdf and Sox9a2 re-established masculinity in XY-Dmy-KD transgenics, insinuating that Dmy initiates masculinity by stimulating and suppressing Gsdf/Sox9a2 and Rspo1 expression, respectively. Gonadal expression of Wt1a starts prior to Dmy and didn't change upon Dmy-KD. Furthermore, Wt1a stimulated the promoter activity of Dmy, suggesting Wt1a as a regulator of Dmy. These findings provide new insights into the role of vertebrate sex-determining genes associated with the molecular interplay between the male and female pathways. |
| 巻・号 | 6 |
| ページ | 19480 |
| 公開日 | 2016-1-25 |
| DOI | 10.1038/srep19480 |
| PII | srep19480 |
| PMID | 26806354 |
| PMC | PMC4726206 |
| MeSH | Amino Acid Sequence / genetics Animals Animals, Genetically Modified Female Fish Proteins / biosynthesis Fish Proteins / genetics* Gene Expression Regulation, Developmental* Gonads / growth & development Male Mutation Oryzias / genetics* Oryzias / growth & development SOX9 Transcription Factor / genetics Sex Determination Processes / genetics* Thrombospondins / genetics Transforming Growth Factor beta / genetics WT1 Proteins / genetics Y Chromosome / genetics |
| IF | 3.998 |
| 引用数 | 20 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | MT828 Qurt TG870 Tg(olvas-GFP) |