RRC ID 36423
Author Chakraborty T, Zhou LY, Chaudhari A, Iguchi T, Nagahama Y.
Title Dmy initiates masculinity by altering Gsdf/Sox9a2/Rspo1 expression in medaka (Oryzias latipes).
Journal Sci Rep
Abstract Despite identification of several sex-determining genes in non-mammalian vertebrates, their detailed molecular cascades of sex determination/differentiation are not known. Here, we used a novel RNAi to characterise the molecular mechanism of Dmy (the sex-determining gene of medaka)-mediated masculinity in XY fish. Dmy knockdown (Dmy-KD) suppressed male pathway (Gsdf, Sox9a2, etc.) and favoured female cascade (Rspo1, etc.) in embryonic XY gonads, resulting in a fertile male-to-female sex-reversal. Gsdf, Sox9a2, and Rspo1 directly interacted with Dmy, and co-injection of Gsdf and Sox9a2 re-established masculinity in XY-Dmy-KD transgenics, insinuating that Dmy initiates masculinity by stimulating and suppressing Gsdf/Sox9a2 and Rspo1 expression, respectively. Gonadal expression of Wt1a starts prior to Dmy and didn't change upon Dmy-KD. Furthermore, Wt1a stimulated the promoter activity of Dmy, suggesting Wt1a as a regulator of Dmy. These findings provide new insights into the role of vertebrate sex-determining genes associated with the molecular interplay between the male and female pathways.
Volume 6
Pages 19480
Published 2016-1-25
DOI 10.1038/srep19480
PII srep19480
PMID 26806354
PMC PMC4726206
MeSH Amino Acid Sequence / genetics Animals Animals, Genetically Modified Female Fish Proteins / biosynthesis Fish Proteins / genetics* Gene Expression Regulation, Developmental* Gonads / growth & development Male Mutation Oryzias / genetics* Oryzias / growth & development SOX9 Transcription Factor / genetics Sex Determination Processes / genetics* Thrombospondins / genetics Transforming Growth Factor beta / genetics WT1 Proteins / genetics Y Chromosome / genetics
IF 3.998
Times Cited 20
Medaka MT828 Qurt TG870 Tg(olvas-GFP)