RRC ID 36424
Author Zhou L, Charkraborty T, Zhou Q, Mohapatra S, Nagahama Y, Zhang Y.
Title Rspo1-activated signalling molecules are sufficient to induce ovarian differentiation in XY medaka (Oryzias latipes).
Journal Sci Rep
Abstract In contrast to our understanding of testicular differentiation, ovarian differentiation is less well understood in vertebrates. In mammals, R-spondin1 (Rspo1), an activator of Wnt/β-catenin signaling pathway, is located upstream of the female sex determination pathway. However, the functions of Rspo1 in ovarian differentiation remain unclear in non-mammalian species. In order to elucidate the detailed functions of Rspo/Wnt signaling pathway in fish sex determination/differentiation, the ectopic expression of the Rspo1 gene was performed in XY medaka (Oryzias latipes). The results obtained demonstrated that the gain of Rspo1 function induced femininity in XY fish. The overexpression of Rspo1 enhanced Wnt4b and β-catenin transcription, and completely suppressed the expression of male-biased genes (Dmy, Gsdf, Sox9a2 and Dmrt1) as well as testicular differentiation. Gonadal reprograming of Rspo1-over-expressed-XY (Rspo1-OV-XY) fish, induced the production of female-biased genes (Cyp19a1a and Foxl2), estradiol-17β production and further female type secondary sexuality. Moreover, Rspo1-OV-XY females were fertile and produced successive generations. Promoter analyses showed that Rspo1 transcription was directly regulated by DM domain genes (Dmy, the sex-determining gene, and Dmrt1) and remained unresponsive to Foxl2. Taken together, our results strongly suggest that Rspo1 is sufficient to activate ovarian development and plays a decisive role in the ovarian differentiation in medaka.
Volume 6
Pages 19543
Published 2016-1-19
DOI 10.1038/srep19543
PII srep19543
PMID 26782368
PMC PMC4726049
MeSH Animals Cell Differentiation / genetics Cell Differentiation / physiology* Female Fish Proteins / genetics* Gene Expression Regulation, Developmental / genetics Male Oryzias / genetics* Oryzias / growth & development* Oryzias / physiology Ovary / physiology* Promoter Regions, Genetic / genetics Sex Determination Processes / genetics Sex Determination Processes / physiology Sex Differentiation / genetics* Sex Differentiation / physiology Testis / physiology Transcription, Genetic / genetics Wnt4 Protein / genetics beta Catenin / genetics
IF 4.122
Times Cited 1
WOS Category MULTIDISCIPLINARY SCIENCES
Resource
Medaka Qurt (MT828)