RRC ID 36433
Author Ng GH, Xu H, Pi N, Kelly BC, Gong Z.
Title Differential GFP expression patterns induced by different heavy metals in Tg(hsp70:gfp) transgenic medaka (Oryzias latipes).
Journal Mar. Biotechnol.
Abstract Heat shock protein 70 (Hsp70) is one of the most widely used biomarker for monitoring environment perturbations in biological systems. To facilitate the analysis of hsp70 expression as a biomarker, we generated a Tg(hsp70:gfp) transgenic medaka line in which green fluorescence protein (GFP) reporter gene was driven by the medaka hsp70 promoter. Here, we characterized Tg(hsp70:gfp) medaka for inducible GFP expression by seven environment-relevant heavy metals, including mercury, arsenic, lead, cadmium, copper, chromium, and zinc. We found that four of them (mercury, arsenic, lead, and cadmium) induced GFP expression in multiple and different organs. In general, the liver, kidney, gut, and skin are among the most frequent organs to show induced GFP expression. In contrast, no detectable GFP induction was observed to copper, chromium, or zinc, indicating that the transgenic line was not responsive to all heavy metals. RT-qPCR determination of hsp70 mRNA showed similar induction and non-induction by these metals, which also correlated with the levels of metal uptake in medaka exposed to these metals. Our observations suggested that these heavy metals have different mechanisms of toxicity and/or differential bioaccumulation in various organs; different patterns of GFP expression induced by different metals may be used to determine or exclude metals in water samples tested. Furthermore, we also tested several non-metal toxicants such as bisphenol A, 2,3,7,8-tetrachlorodibenzo-p-dioxin, 4-introphenol, and lindane; none of them induced significant GFP expression in Tg(hsp70:gfp) medaka, further suggesting that the inducibility of Tg(hsp70:gfp) for GFP expression is specific to a subset of heavy metals.
Volume 17(3)
Pages 317-27
Published 2015-6
DOI 10.1007/s10126-015-9620-5
PMID 25652692
MeSH Animals Animals, Genetically Modified Benzhydryl Compounds / toxicity Female Fish Proteins / agonists Fish Proteins / genetics* Fish Proteins / metabolism Gene Expression Regulation Genes, Reporter Green Fluorescent Proteins / agonists Green Fluorescent Proteins / genetics* Green Fluorescent Proteins / metabolism HSP70 Heat-Shock Proteins / agonists HSP70 Heat-Shock Proteins / genetics* HSP70 Heat-Shock Proteins / metabolism Intestines / drug effects Intestines / metabolism Kidney / drug effects Kidney / metabolism Lindane / toxicity Liver / drug effects Liver / metabolism Male Metals, Heavy / toxicity* Nitrophenols / toxicity Oryzias / genetics* Oryzias / metabolism Phenols / toxicity Polychlorinated Dibenzodioxins / toxicity Promoter Regions, Genetic RNA, Messenger / genetics RNA, Messenger / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Skin / drug effects Skin / metabolism Water Pollutants, Chemical / toxicity*
IF 2.328
Times Cited 0
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY MARINE & FRESHWATER BIOLOGY
Resource
Medaka HdrR-II1 (IB178)