論文 - 詳細
| RRC ID | 36451 |
|---|---|
| 著者 | Watakabe A, Ohtsuka M, Kinoshita M, Takaji M, Isa K, Mizukami H, Ozawa K, Isa T, Yamamori T. |
| タイトル | Comparative analyses of adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. |
| ジャーナル | Neurosci Res |
| Abstract |
Here we investigated the transduction characteristics of adeno-associated viral vector (AAV) serotypes 1, 2, 5, 8 and 9 in the marmoset cerebral cortex. Using three constructs that each has hrGFP under ubiquitous (CMV), or neuron-specific (CaMKII and Synapsin I (SynI)) promoters, we investigated (1) the extent of viral spread, (2) cell type tropism, and (3) neuronal transduction efficiency of each serotype. AAV2 was clearly distinct from other serotypes in small spreading and neuronal tropism. We did not observe significant differences in viral spread among other serotypes. Regarding the cell tropism, AAV1, 5, 8 and 9 exhibited mostly glial expression for CMV construct. However, when the CaMKII construct was tested, cortical neurons were efficiently transduced (>∼70% in layer 3) by all serotypes, suggesting that glial expression obscured neuronal expression for CMV construct. For both SynI and CaMKII constructs, we observed generally high-level expression in large pyramidal cells especially in layer 5, as well as in parvalbumin-positive interneurons. The expression from the CaMKII construct was more uniformly observed in excitatory cells compared with SynI construct. Injection of the same viral preparations in mouse and macaque cortex resulted in essentially the same result with some species-specific differences. |
| 巻・号 | 93 |
| ページ | 144-57 |
| 公開日 | 2015-4-1 |
| DOI | 10.1016/j.neures.2014.09.002 |
| PII | S0168-0102(14)00213-2 |
| PMID | 25240284 |
| MeSH | Animals Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism Callithrix Cerebral Cortex / cytology Cerebral Cortex / metabolism* Dependovirus / genetics* Female Genetic Vectors Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Macaca Male Mice, Inbred C57BL Neurons / metabolism Promoter Regions, Genetic Species Specificity Synapsins / genetics Synapsins / metabolism Transduction, Genetic |
| IF | 2.645 |
| 引用数 | 99 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 50 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ニホンザル | |