RRC ID 36467
Author Nonomura K, Miyoshi K, Eiguchi M, Suzuki T, Miyao A, Hirochika H, Kurata N.
Title The MSP1 gene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice.
Journal Plant Cell
Abstract The function of the novel gene MSP1 (MULTIPLE SPOROCYTE), which controls early sporogenic development, was elucidated by characterizing a retrotransposon-tagged mutation of rice. The MSP1 gene encoded a Leu-rich repeat receptor-like protein kinase. The msp1 mutation gave rise to an excessive number of both male and female sporocytes. In addition, the formation of anther wall layers was disordered and the tapetum layer was lost completely. Although the mutation never affected homologous chromosome pairing and chiasma maintenance, the development of pollen mother cells was arrested at various stages of meiotic prophase I, which resulted in complete male sterility. Meanwhile, plural megaspore mother cells in a mutant ovule generated several megaspores, underwent gametogenesis, and produced germinable seeds when fertilized with wild-type pollen despite disorganized female gametophytes. In situ expression of MSP1 was detected in surrounding cells of male and female sporocytes and some flower tissues, but never in the sporocytes themselves. These results suggest that the MSP1 product plays crucial roles in restricting the number of cells entering into male and female sporogenesis and in initiating anther wall formation in rice.
Volume 15(8)
Pages 1728-39
Published 2003-8-1
DOI 10.1105/tpc.012401
PMID 12897248
PMC PMC167165
MeSH Amino Acid Sequence Arabidopsis / genetics Base Sequence DNA, Plant / genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant* Meiosis Molecular Sequence Data Mutation Oryza / cytology Oryza / genetics* Oryza / growth & development Oryza / physiology Protein-Serine-Threonine Kinases / genetics Sequence Homology, Amino Acid Spores / cytology Zea mays / genetics
IF 8.631
Times Cited 182
WOS Category PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Rice Sterile strain (STER-NIG0001 (MSP1-1), STER-NIG0002 (MSP1-2), STER-NIG0003 (MSP1-3))