RRC ID 36469
Author Nonomura KI, Nakano M, Murata K, Miyoshi K, Eiguchi M, Miyao A, Hirochika H, Kurata N.
Title An insertional mutation in the rice PAIR2 gene, the ortholog of Arabidopsis ASY1, results in a defect in homologous chromosome pairing during meiosis.
Journal Mol Genet Genomics
Abstract To elucidate the genetic system that establishes homologous chromosome pairing in monocot plants, we have isolated an asynaptic mutant of rice, designated pair2 (homologous pairing aberration in rice meiosis 2), in which 24 completely unpaired univalents are observed at pachytene and diakinesis. The mutation was caused by an insertion of the retrotransposon Tos17, as demonstrated by complementation of the mutation by transformation with the corresponding wild-type gene. The gene in which the element was inserted is orthologous to the ASY1 gene of Arabidopsis thaliana and the HOP1 gene of Saccharomyces cerevisiae. Mature PAIR2 mRNA and several splicing variants were found to be highly expressed in wild-type reproductive tissues, and lower expression was also detected in vegetative tissues. In situ hybridization and BrdU incorporation experiments revealed that PAIR2 expression is specifically enhanced in male and female meiocytes, but not in those at pre-meiotic S phase or in the pollen maturation stages. The results obtained in this study suggest that the PAIR2 gene is essential for homologous chromosome pairing in meiosis, as in the case of the genes ASY1 and HOP1. The study also suggested the possibility that a highly homologous copy of the PAIR2 gene located on a different chromosome is in fact a pseudogene.
Volume 271(2)
Pages 121-9
Published 2004-3-1
DOI 10.1007/s00438-003-0934-z
PMID 14758540
MeSH Amino Acid Sequence Bromodeoxyuridine Chromosome Pairing / genetics* DNA Primers Gene Expression Profiling* In Situ Hybridization Meiosis / genetics* Molecular Sequence Data Mutation / genetics Oryza / genetics* RNA, Messenger / genetics Retroelements / genetics* Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment
IF 2.879
Times Cited 69
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Rice Sterile strain (STER-NIG0007) (PAIR2)