論文 - 詳細
| RRC ID | 36504 |
|---|---|
| 著者 | Cherif MS, Shuaibu MN, Kodama Y, Kurosaki T, Helegbe GK, Kikuchi M, Ichinose A, Yanagi T, Sasaki H, Yui K, Tien NH, Karbwang J, Hirayama K. |
| タイトル | Nanoparticle formulation enhanced protective immunity provoked by PYGPI8p-transamidase related protein (PyTAM) DNA vaccine in Plasmodium yoelii malaria model. |
| ジャーナル | Vaccine |
| Abstract |
We have previously reported the new formulation of polyethylimine (PEI) with gamma polyglutamic acid (γ-PGA) nanoparticle (NP) to have provided Plasmodium yoelii merozoite surface protein-1 (PyMSP-1) plasmid DNA vaccine with enhanced protective cellular and humoral immunity in the lethal mouse malaria model. PyGPI8p-transamidase-related protein (PyTAM) was selected as a possible candidate vaccine antigen by using DNA vaccination screening from 29 GPI anchor and signal sequence motif positive genes picked up using web-based bioinformatics tools; though the observed protection was not complete. Here, we observed augmented protective effect of PyTAM DNA vaccine by using PEI and γ-PGA complex as delivery system. NP-coated PyTAM plasmid DNA immunized mice showed a significant survival rate from lethal P. yoelii challenge infection compared with naked PyTAM plasmid or with NP-coated empty plasmid DNA group. Antigen-specific IgG1 and IgG2b subclass antibody levels, proportion of CD4 and CD8T cells producing IFN-γ in the splenocytes and IL-4, IFN-γ, IL-12 and TNF-α levels in the sera and in the supernatants from ex vivo splenocytes culture were all enhanced by the NP-coated PyTAM DNA vaccine. These data indicates that NP augments PyTAM protective immune response, and this enhancement was associated with increased DC activation and concomitant IL-12 production. |
| 巻・号 | 32(17) |
| ページ | 1998-2006 |
| 公開日 | 2014-4-7 |
| DOI | 10.1016/j.vaccine.2014.01.005 |
| PII | S0264-410X(14)00006-1 |
| PMID | 24440206 |
| MeSH | Animals Antibodies, Protozoan / blood Cells, Cultured Dendritic Cells / immunology Female Immunity, Cellular Immunity, Humoral Interferon-gamma / immunology Interleukin-12 / immunology Malaria / prevention & control* Malaria Vaccines / genetics Malaria Vaccines / immunology* Mice Mice, Inbred C57BL Nanoparticles* Plasmodium yoelii / immunology* Polyethyleneimine / chemistry Polyglutamic Acid / analogs & derivatives Polyglutamic Acid / chemistry Protozoan Proteins / immunology* Recombinant Proteins / immunology Spleen / cytology Spleen / immunology T-Lymphocytes / immunology Vaccines, DNA / immunology |
| IF | 3.143 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | |