| RRC ID |
36504
|
| 著者 |
Cherif MS, Shuaibu MN, Kodama Y, Kurosaki T, Helegbe GK, Kikuchi M, Ichinose A, Yanagi T, Sasaki H, Yui K, Tien NH, Karbwang J, Hirayama K.
|
| タイトル |
Nanoparticle formulation enhanced protective immunity provoked by PYGPI8p-transamidase related protein (PyTAM) DNA vaccine in Plasmodium yoelii malaria model.
|
| ジャーナル |
Vaccine
|
| Abstract |
We have previously reported the new formulation of polyethylimine (PEI) with gamma polyglutamic acid (γ-PGA) nanoparticle (NP) to have provided Plasmodium yoelii merozoite surface protein-1 (PyMSP-1) plasmid DNA vaccine with enhanced protective cellular and humoral immunity in the lethal mouse malaria model. PyGPI8p-transamidase-related protein (PyTAM) was selected as a possible candidate vaccine antigen by using DNA vaccination screening from 29 GPI anchor and signal sequence motif positive genes picked up using web-based bioinformatics tools; though the observed protection was not complete. Here, we observed augmented protective effect of PyTAM DNA vaccine by using PEI and γ-PGA complex as delivery system. NP-coated PyTAM plasmid DNA immunized mice showed a significant survival rate from lethal P. yoelii challenge infection compared with naked PyTAM plasmid or with NP-coated empty plasmid DNA group. Antigen-specific IgG1 and IgG2b subclass antibody levels, proportion of CD4 and CD8T cells producing IFN-γ in the splenocytes and IL-4, IFN-γ, IL-12 and TNF-α levels in the sera and in the supernatants from ex vivo splenocytes culture were all enhanced by the NP-coated PyTAM DNA vaccine. These data indicates that NP augments PyTAM protective immune response, and this enhancement was associated with increased DC activation and concomitant IL-12 production.
|
| 巻・号 |
32(17)
|
| ページ |
1998-2006
|
| 公開日 |
2014-4-7
|
| DOI |
10.1016/j.vaccine.2014.01.005
|
| PII |
S0264-410X(14)00006-1
|
| PMID |
24440206
|
| MeSH |
Animals
Antibodies, Protozoan / blood
Cells, Cultured
Dendritic Cells / immunology
Female
Immunity, Cellular
Immunity, Humoral
Interferon-gamma / immunology
Interleukin-12 / immunology
Malaria / prevention & control*
Malaria Vaccines / genetics
Malaria Vaccines / immunology*
Mice
Mice, Inbred C57BL
Nanoparticles*
Plasmodium yoelii / immunology*
Polyethyleneimine / chemistry
Polyglutamic Acid / analogs & derivatives
Polyglutamic Acid / chemistry
Protozoan Proteins / immunology*
Recombinant Proteins / immunology
Spleen / cytology
Spleen / immunology
T-Lymphocytes / immunology
Vaccines, DNA / immunology
|
| IF |
3.143
|
| 引用数 |
10
|
| リソース情報 |
| 病原微生物 |
|
