| RRC ID |
36505
|
| 著者 |
Bao LQ, Huy NT, Kikuchi M, Yanagi T, Senba M, Shuaibu MN, Honma K, Yui K, Hirayama K.
|
| タイトル |
CD19(+) B cells confer protection against experimental cerebral malaria in semi-immune rodent model.
|
| ジャーナル |
PLoS One
|
| Abstract |
In African endemic area, adults are less vulnerable to cerebral malaria than children probably because of acquired partial immunity or semi-immune status. Here, we developed an experimental cerebral malaria (ECM) model for semi-immune mice. C57BL/6 (B6) mice underwent one, two and three cycles of infection and radical treatment (1-cure, 2-cure and 3-cure, respectively) before being finally challenged with 10(4) Plasmodium berghei ANKA without treatment. Our results showed that 100% of naïve (0-cure), 67% of 1-cure, 37% of 2-cure and none of 3-cure mice succumbed to ECM within 10 days post challenge infection. In the protected 3-cure mice, significantly higher levels of plasma IL-10 and lower levels of IFN-γ than the others on day 7 post challenge infection were observed. Major increased lymphocyte subset of IL-10 positive cells in 3-cure mice was CD5(-)CD19(+) B cells. Passive transfer of splenic CD19(+) cells from 3-cure mice protected naïve mice from ECM. Additionally, aged 3-cure mice were also protected from ECM 12 and 20 months after the last challenge infection. In conclusion, mice became completely resistant to ECM after three exposures to malaria. CD19(+) B cells are determinants in protective mechanism of semi-immune mice against ECM possibly via modulatory IL-10 for pathogenic IFN-γ production.
|
| 巻・号 |
8(5)
|
| ページ |
e64836
|
| 公開日 |
2013-1-1
|
| DOI |
10.1371/journal.pone.0064836
|
| PII |
PONE-D-12-36336
|
| PMID |
23724100
|
| PMC |
PMC3665539
|
| MeSH |
Adoptive Transfer
Animals
Antibodies, Protozoan / blood
Antigens, CD19 / metabolism*
B-Lymphocytes / immunology*
CD5 Antigens / metabolism
Disease Models, Animal
Disease Resistance / immunology
Disease Susceptibility
Immunoglobulin G / blood
Interferon-gamma / blood
Interleukin-10 / biosynthesis
Interleukin-10 / blood
Malaria, Cerebral / blood
Malaria, Cerebral / immunology*
Malaria, Cerebral / parasitology
Malaria, Cerebral / prevention & control*
Mice
Mice, Inbred C57BL
Parasitemia / immunology
Parasitemia / parasitology
Plasmodium berghei / physiology
Species Specificity
Spleen / pathology
|
| IF |
2.74
|
| 引用数 |
9
|
| リソース情報 |
| 病原微生物 |
|
