| RRC ID |
36542
|
| 著者 |
Araki T, Tsujioka M, Abe T, Fukuzawa M, Meima M, Schaap P, Morio T, Urushihara H, Katoh M, Maeda M, Tanaka Y, Takeuchi I, Williams JG.
|
| タイトル |
A STAT-regulated, stress-induced signalling pathway in Dictyostelium.
|
| ジャーナル |
J Cell Sci
|
| Abstract |
The Dictyostelium stalk cell inducer differentiation-inducing factor (DIF) directs tyrosine phosphorylation and nuclear accumulation of the STAT (signal transducer and activator of transcription) protein Dd-STATc. We show that hyperosmotic stress, heat shock and oxidative stress also activate Dd-STATc. Hyperosmotic stress is known to elevate intracellular cGMP and cAMP levels, and the membrane-permeant analogue 8-bromo-cGMP rapidly activates Dd-STATc, whereas 8-bromo-cAMP is a much less effective inducer. Surprisingly, however, Dd-STATc remains stress activatable in null mutants for components of the known cGMP-mediated and cAMP-mediated stress-response pathways and in a double mutant affecting both pathways. Also, Dd-STATc null cells are not abnormally sensitive to hyperosmotic stress. Microarray analysis identified two genes, gapA and rtoA, that are induced by hyperosmotic stress. Osmotic stress induction of gapA and rtoA is entirely dependent on Dd-STATc. Neither gene is inducible by DIF but both are rapidly inducible with 8-bromo-cGMP. Again, 8-bromo-cAMP is a much less potent inducer than 8-bromo-cGMP. These data show that Dd-STATc functions as a transcriptional activator in a stress-response pathway and the pharmacological evidence, at least, is consistent with cGMP acting as a second messenger.
|
| 巻・号 |
116(Pt 14)
|
| ページ |
2907-15
|
| 公開日 |
2003-7-15
|
| DOI |
10.1242/jcs.00501
|
| PII |
jcs.00501
|
| PMID |
12771188
|
| MeSH |
Active Transport, Cell Nucleus
Animals
Blotting, Northern
Blotting, Western
Cell Cycle Proteins / metabolism
Cell Nucleus / metabolism
Cyclic AMP / metabolism
Cyclic GMP / analogs & derivatives*
Cyclic GMP / metabolism
Dictyostelium / metabolism*
Dose-Response Relationship, Drug
Green Fluorescent Proteins
Immunohistochemistry
Luminescent Proteins / metabolism
Models, Biological
Mutation
Oligonucleotide Array Sequence Analysis
Osmosis
Oxidative Stress
Phosphorylation
Protein Transport
Protozoan Proteins / metabolism
Protozoan Proteins / physiology*
STAT Transcription Factors
Signal Transduction*
Time Factors
Trans-Activators / metabolism
Trans-Activators / physiology*
Transcription, Genetic
Tyrosine / metabolism
|
| IF |
4.573
|
| 引用数 |
34
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
|
