RRC ID 36594
Author Sillo A, Matthias J, Konertz R, Bozzaro S, Eichinger L.
Title Salmonella typhimurium is pathogenic for Dictyostelium cells and subverts the starvation response.
Journal Cell Microbiol
Abstract In unicellular amoebae, such as Dictyostelium discoideum, bacterial phagocytosis is a food hunting device, while in higher organisms it is the first defence barrier against microbial infection. In both cases, pathogenic bacteria exploit phagocytosis to enter the cell and multiply intracellularly. Salmonella typhimurium, the agent of food-borne gastroenteritis, is phagocytosed by both macrophages and Dictyostelium cells. By using cell biological assays and global transcriptional analysis with DNA microarrays covering the Dictyostelium genome, we show here that S. typhimurium is pathogenic for Dictyostelium cells. Depending on the degree of virulence, which in turn depended on bacterial growth conditions, Salmonella could kill Dictyostelium cells or inhibit their growth and development. In the early phase of infection in non-nutrient buffer, the ingested bacteria escaped degradation, induced a starvation-like transcriptional response but inhibited selectively genes required for chemotaxis and aggregation. This way differentiation of the host cells into spore and stalk cells was blocked or delayed, which in turn is likely to be favourable for the establishment of a replicative niche for Salmonella. Inhibition of the aggregation competence and chemotactic streaming of aggregation-competent cells in the presence of Salmonella suggests interference with cAMP signalling.
Volume 13(11)
Pages 1793-811
Published 2011-11-1
DOI 10.1111/j.1462-5822.2011.01662.x
PMID 21824247
MeSH Cell Survival Cyclic AMP / metabolism Dictyostelium / growth & development Dictyostelium / microbiology* Dictyostelium / physiology* Gene Expression Profiling Microarray Analysis Phagocytosis* Salmonella typhimurium / growth & development Salmonella typhimurium / pathogenicity* Signal Transduction
IF 3.43
Times Cited 17
Cellular slime molds