RRC ID 36645
著者 Sato Y, Nagata S, Takiguchi M.
タイトル Effective elicitation of human effector CD8+ T Cells in HLA-B*51:01 transgenic humanized mice after infection with HIV-1.
ジャーナル PLoS One
Abstract Humanized mice are expected to be useful as small animal models for in vivo studies on the pathogenesis of infectious diseases. However, it is well known that human CD8(+) T cells cannot differentiate into effector cells in immunodeficient mice transplanted with only human CD34(+) hematopoietic stem cells (HSCs), because human T cells are not educated by HLA in the mouse thymus. We here established HLA-B*51:01 transgenic humanized mice by transplanting human CD34(+) HSCs into HLA-B*51:01 transgenic NOD/SCID/Jak3(-/-) mice (hNOK/B51Tg mice) and investigated whether human effector CD8(+) T cells would be elicited in the mice or in those infected with HIV-1 NL4-3. There were no differences in the frequency of late effector memory and effector subsets (CD27(low)CD28(-)CD45RA(+/-)CCR7(-) and CD27(-)CD28(-)CD45RA(+/-)CCR7(-), respectively) among human CD8(+) T cells and in that of human CD8(+) T cells expressing CX3CR1 and/or CXCR1 between hNOK/B51Tg and hNOK mice. In contrast, the frequency of late effector memory and effector CD8(+) T cell subsets and of those expressing CX3CR1 and/or CXCR1 was significantly higher in HIV-1-infected hNOK/B51Tg mice than in uninfected ones, whereas there was no difference in that of these subsets between HIV-1-infected and uninfected hNOK mice. These results suggest that hNOK/B51Tg mice had CD8(+) T cells that were capable of differentiating into effector T cells after viral antigen stimulation and had a greater ability to elicit effector CD8(+) T cells than hNOK ones.
巻・号 7(8)
ページ e42776
公開日 2012-1-1
DOI 10.1371/journal.pone.0042776
PII PONE-D-12-10664
PMID 22880104
PMC PMC3412802
MeSH Animals CD8-Positive T-Lymphocytes / immunology* CX3C Chemokine Receptor 1 Disease Models, Animal HIV Infections / immunology* HIV-1 / immunology* HLA-B51 Antigen / immunology* Humans Janus Kinase 3 / deficiency Janus Kinase 3 / metabolism Mice Mice, Inbred NOD Mice, SCID Mice, Transgenic Phenotype Receptors, Chemokine / immunology Receptors, Interleukin-8A / immunology
IF 2.74
引用数 7
WOS 分野 IMMUNOLOGY
リソース情報
研究用ヒト臍帯血幹細胞 研究用ヒト臍帯血幹細胞