論文 - 詳細
| RRC ID | 36645 |
|---|---|
| 著者 | Sato Y, Nagata S, Takiguchi M. |
| タイトル | Effective elicitation of human effector CD8+ T Cells in HLA-B*51:01 transgenic humanized mice after infection with HIV-1. |
| ジャーナル | PLoS One |
| Abstract |
Humanized mice are expected to be useful as small animal models for in vivo studies on the pathogenesis of infectious diseases. However, it is well known that human CD8(+) T cells cannot differentiate into effector cells in immunodeficient mice transplanted with only human CD34(+) hematopoietic stem cells (HSCs), because human T cells are not educated by HLA in the mouse thymus. We here established HLA-B*51:01 transgenic humanized mice by transplanting human CD34(+) HSCs into HLA-B*51:01 transgenic NOD/SCID/Jak3(-/-) mice (hNOK/B51Tg mice) and investigated whether human effector CD8(+) T cells would be elicited in the mice or in those infected with HIV-1 NL4-3. There were no differences in the frequency of late effector memory and effector subsets (CD27(low)CD28(-)CD45RA(+/-)CCR7(-) and CD27(-)CD28(-)CD45RA(+/-)CCR7(-), respectively) among human CD8(+) T cells and in that of human CD8(+) T cells expressing CX3CR1 and/or CXCR1 between hNOK/B51Tg and hNOK mice. In contrast, the frequency of late effector memory and effector CD8(+) T cell subsets and of those expressing CX3CR1 and/or CXCR1 was significantly higher in HIV-1-infected hNOK/B51Tg mice than in uninfected ones, whereas there was no difference in that of these subsets between HIV-1-infected and uninfected hNOK mice. These results suggest that hNOK/B51Tg mice had CD8(+) T cells that were capable of differentiating into effector T cells after viral antigen stimulation and had a greater ability to elicit effector CD8(+) T cells than hNOK ones. |
| 巻・号 | 7(8) |
| ページ | e42776 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0042776 |
| PII | PONE-D-12-10664 |
| PMID | 22880104 |
| PMC | PMC3412802 |
| MeSH | Animals CD8-Positive T-Lymphocytes / immunology* CX3C Chemokine Receptor 1 Disease Models, Animal HIV Infections / immunology* HIV-1 / immunology* HLA-B51 Antigen / immunology* Humans Janus Kinase 3 / deficiency Janus Kinase 3 / metabolism Mice Mice, Inbred NOD Mice, SCID Mice, Transgenic Phenotype Receptors, Chemokine / immunology Receptors, Interleukin-8A / immunology |
| IF | 2.74 |
| 引用数 | 7 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 研究用ヒト臍帯血幹細胞 | 研究用ヒト臍帯血幹細胞 |