RRC ID 36675
Author Shimazu T, Mori Y, Takahashi A, Tsunoda H, Tojo A, Nagamura-Inoue T.
Title Serum- and xeno-free cryopreservation of human umbilical cord tissue as mesenchymal stromal cell source.
Journal Cytotherapy
Abstract BACKGROUND AIMS:Human umbilical cord (UC) has become a notable source for mesenchymal stromal cells (MSCs) that can migrate to areas of inflammation and damaged tissue and can suppress excess immune reactions and to repair, respectively. Although UC is a solid tissue, there are several advantages, including repeatable uses from the same donor sample when needed and the possibility of future explorations for cells with unknown potential, if we could cryopreserve the UC as a living tissue material. However, because the cryoprotectants in the previous reports included animal- or allogeneic human-derived serum or no serum, the frozen-thawed UC-MSCs were inferior to fresh UC-MSCs in cell proliferation. The objective of this study was to find a suitable cryopreservation method of UC for clinical use.
METHODS:The UC was cut in cross-section and incised longitudinally, immersed in the cryoprotectant and frozen slowly. Later, it was thawed and minced rapidly, and the fragments of UC were cultured by improved explant method.
RESULTS:The highest yield of cells was obtained from frozen-thawed UC with serum- and xeno-free cryoprotectant, STEM-CELLBANKER, when compared with others. The cells derived from frozen-thawed UC stored in STEM-CELLBANKER expressed the phenotypes of MSCs, retained the immunosuppressive properties in allogeneic mixed lymphocyte reactions and the differentiation potentials (into adipocyte and chondrocytes) comparable to those derived from fresh UC.
CONCLUSIONS:UC can be cryopreserved in serum- and xeno-free cryoprotectant as a living tissue while keeping its growth and functions equivalent to fresh UC. Our method is simple and feasible for clinical use.
Volume 17(5)
Pages 593-600
Published 2015-5-1
DOI 10.1016/j.jcyt.2015.03.604
PII S1465-3249(15)00688-X
PMID 25881518
MeSH Adipocytes / cytology Adipocytes / drug effects Animals Biomarkers / metabolism Cell Count Cell Differentiation / drug effects Cell Differentiation / genetics Cell Proliferation / drug effects Cell Separation Cells, Cultured Chondrocytes / cytology Chondrocytes / drug effects Chondrocytes / metabolism Cryopreservation / methods* Cryoprotective Agents / pharmacology Culture Media, Serum-Free Freezing Humans Immunosuppression Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / drug effects Mesenchymal Stem Cells / metabolism Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / metabolism T-Lymphocytes / cytology T-Lymphocytes / drug effects Umbilical Cord / cytology*
IF 4.218
Times Cited 13
WOS Category MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY HEMATOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY
Resource
Cord blood stem cells for research 研究用ヒト臍帯血幹細胞