| RRC ID |
36759
|
| Author |
Kaneko K, Higuchi C, Kunugiza Y, Yoshida K, Sakai T, Yoshikawa H, Nakata K.
|
| Title |
Hyaluronan inhibits BMP-induced osteoblast differentiation.
|
| Journal |
FEBS Lett
|
| Abstract |
Hyaluronan (HA), one of the major structural extracellular components in cartilage, regulates cellular responses via receptors such as CD44. However, the direct effects of HA on osteoblastic differentiation has not been studied in detail. Here, we investigated the effects of HA (molecular weight: 900-1200 kDa) on osteoblastic differentiation that was induced by bone morphogenetic protein (BMP) in C2C12 cells (mouse myoblastic cells) and ST2 cells (mouse bone marrow cells). BMP-induced osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation were downregulated by HA. Use of the CD44-blocking antibody restored HA-induced inhibition of osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation. Our results indicate that HA inhibits BMP-induced osteoblastic differentiation through the CD44 receptor.
|
| Volume |
589(4)
|
| Pages |
447-54
|
| Published |
2015-2-13
|
| DOI |
10.1016/j.febslet.2014.12.031
|
| PII |
S0014-5793(15)00005-8
|
| PMID |
25592835
|
| MeSH |
Adult
Aged
Alkaline Phosphatase / metabolism
Animals
Bone Morphogenetic Protein 2 / physiology*
Cell Differentiation*
Cell Line
Female
Humans
Hyaluronan Receptors / metabolism
Hyaluronic Acid / pharmacology*
Hyaluronic Acid / physiology
Male
Mice
Middle Aged
Osteoarthritis / metabolism
Osteoarthritis / pathology
Osteoblasts / physiology*
Restriction Mapping
rho GTP-Binding Proteins / metabolism
rhoA GTP-Binding Protein
|
| IF |
3.057
|
| Times Cited |
17
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987)
ST2(RCB0224) |
