| RRC ID |
36765
|
| Author |
Lai YT, Sato M, Ohta S, Akamatsu K, Nakao S, Sakai Y, Ito T.
|
| Title |
Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique.
|
| Journal |
Colloids Surf B Biointerfaces
|
| Abstract |
We have developed a new type of artificial oxygen carrier composed of bovine hemoglobin (bHb) and bovine serum albumin (BSA) prepared by Shirasu porous glass (SPG) membrane emulsification technique. The resultant emulsion droplets containing 10 wt% bHb and 5-20 wt% BSA were subsequently cross-linked by glutaraldehyde to form the microspheres. Due to the uniform pore structure of SPG membranes, the average diameters of bHb10-BSAm microspheres were successfully controlled at around 5 μm with a coefficient of variation of around 10%. In addition, the biocompatibility of the carriers depended on their oxyhemoglobin percentage regardless of their same size. Finally, the P50 values of these microspheres ranged from 8.08 to 11.60 mmHg, which showed a high oxygen affinity and an oxygen delivery function.
|
| Volume |
127
|
| Pages |
1-7
|
| Published |
2015-3-1
|
| DOI |
10.1016/j.colsurfb.2015.01.018
|
| PII |
S0927-7765(15)00025-9
|
| PMID |
25635614
|
| MeSH |
Animals
Cattle
Cell Survival
Drug Compounding / methods*
Emulsions / chemistry*
Glass / chemistry*
Hemoglobins / chemistry*
Hep G2 Cells
Human Umbilical Vein Endothelial Cells
Humans
Membranes, Artificial
Microscopy, Electron, Scanning
Microspheres*
Optical Imaging
Oxygen / chemistry*
Particle Size*
Porosity
Protein Stability
Serum Albumin, Bovine / chemistry*
Shear Strength
Spectrophotometry, Ultraviolet
Viscosity
|
| IF |
4.389
|
| Times Cited |
18
|
|
WOS Category
|
BIOPHYSICS
MATERIALS SCIENCE, BIOMATERIALS
CHEMISTRY, PHYSICAL
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1648)
RAW 264(RCB0535) |
