| RRC ID |
36788
|
| 著者 |
Wakita A, Motoyama S, Sato Y, Koyota S, Usami S, Yoshino K, Sasaki T, Imai K, Saito H, Minamiya Y.
|
| タイトル |
REG Iα activates c-Jun through MAPK pathways to enhance the radiosensitivity of squamous esophageal cancer cells.
|
| ジャーナル |
Tumour Biol
|
| Abstract |
Identification of the key molecules that mediate susceptibility to anticancer treatments would be highly desirable. Based on clinical and cell biological studies, we recently proposed that regenerating gene (REG) Iα may be such a molecule. In the present study, we hypothesized that REG Iα increases radiosensitivity through activation of mitogen-activated protein kinase (MAPK) pathways. To test that idea, we transfected TE-5 and TE-9 squamous esophageal cancer cells with REG Iα and examined its involvement in MAPK signaling and its effect on susceptibility to radiotherapy. We found that REG Iα-expressing cells showed increased expression of c-Jun messenger RNA (mRNA) and phospho-c-Jun protein mediated via the c-Jun N-terminal kinase (JNK) pathway and extracellular signal-regulated kinase (ERK) pathway, as well as increased radiosensitivity. Immunohistochemical analysis confirmed the activation of c-Jun in tumors expressing REG Iα. Collectively, these findings suggest that REG Iα activates c-Jun via the JNK and ERK pathway, thereby enhancing radiosensitivity.
|
| 巻・号 |
36(7)
|
| ページ |
5249-54
|
| 公開日 |
2015-7-1
|
| DOI |
10.1007/s13277-015-3183-y
|
| PII |
10.1007/s13277-015-3183-y
|
| PMID |
25656613
|
| MeSH |
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / pathology
Carcinoma, Squamous Cell / radiotherapy
Cell Line, Tumor
Esophageal Neoplasms / genetics*
Esophageal Neoplasms / pathology
Esophageal Neoplasms / radiotherapy
Esophageal Squamous Cell Carcinoma
Gene Expression Regulation, Neoplastic
Humans
JNK Mitogen-Activated Protein Kinases / biosynthesis
JNK Mitogen-Activated Protein Kinases / genetics*
Lithostathine / genetics*
Lithostathine / metabolism
MAP Kinase Signaling System / genetics
Mitogen-Activated Protein Kinases / genetics
Proto-Oncogene Proteins c-jun / biosynthesis
Proto-Oncogene Proteins c-jun / genetics*
RNA, Messenger / biosynthesis
Radiation Tolerance / genetics
|
| IF |
3.65
|
| 引用数 |
8
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
TE-5(RCB1949)
TE-9(RCB1988 |
