論文 - 詳細
| RRC ID | 36828 |
|---|---|
| 著者 | McCallum KC, Liu B, Fierro-González JC, Swoboda P, Arur S, Miranda-Vizuete A, Garsin DA. |
| タイトル | TRX-1 Regulates SKN-1 Nuclear Localization Cell Non-autonomously in Caenorhabditis elegans. |
| ジャーナル | Genetics |
| Abstract |
The Caenorhabditis elegans oxidative stress response transcription factor, SKN-1, is essential for the maintenance of redox homeostasis and is a functional ortholog of the Nrf family of transcription factors. The numerous levels of regulation that govern these transcription factors underscore their importance. Here, we add a thioredoxin, encoded by trx-1, to the expansive list of SKN-1 regulators. We report that loss of trx-1 promotes nuclear localization of intestinal SKN-1 in a redox-independent, cell non-autonomous fashion from the ASJ neurons. Furthermore, this regulation is not general to the thioredoxin family, as two other C. elegans thioredoxins, TRX-2 and TRX-3, do not play a role in this process. Moreover, TRX-1-dependent regulation requires signaling from the p38 MAPK-signaling pathway. However, while TRX-1 regulates SKN-1 nuclear localization, classical SKN-1 transcriptional activity associated with stress response remains largely unaffected. Interestingly, RNA-Seq analysis revealed that loss of trx-1 elicits a general, organism-wide down-regulation of several classes of genes; those encoding for collagens and lipid transport being most prevalent. Together, these results uncover a novel role for a thioredoxin in regulating intestinal SKN-1 nuclear localization in a cell non-autonomous manner, thereby contributing to the understanding of the processes involved in maintaining redox homeostasis throughout an organism. |
| 巻・号 | 203(1) |
| ページ | 387-402 |
| 公開日 | 2016-5-1 |
| DOI | 10.1534/genetics.115.185272 |
| PII | genetics.115.185272 |
| PMID | 26920757 |
| PMC | PMC4858787 |
| MeSH | Active Transport, Cell Nucleus Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Intestinal Mucosa / metabolism Intestines / cytology Neurons / metabolism Oxidative Stress Signal Transduction* Thioredoxins / genetics Thioredoxins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 4.015 |
| 引用数 | 10 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2720 tm2820 |