RRC ID 36835
著者 Tanaka Y, Nagashima H, Bando K, Lu L, Ozaki A, Morita Y, Fukumoto S, Ishii N, Sugawara S.
タイトル Oral CD103-CD11b+ classical dendritic cells present sublingual antigen and induce Foxp3+ regulatory T cells in draining lymph nodes.
ジャーナル Mucosal Immunol
Abstract Sublingual immunotherapy (SLIT) is a safe and efficient treatment for type 1 allergies; however, the underlying immunological mechanisms, particularly the phenotype of oral antigen-presenting cells (APCs) responsible for the induction of regulatory T (Treg) cells, remain unclear. We show here that the sublingual application of ovalbumin (OVA) induced antigen-specific Foxp3+ Treg cells in draining submandibular lymph nodes (ManLNs). Oral APCs were classified into macrophages, classical dendritic cells (cDCs), and Langerhans cells by flow cytometry. A major subset of oral cDCs with the CD103-CD11b+ phenotype showed retinoic acid (RA)-producing activity and converted naive CD4+ T cells to Foxp3+ Treg cells in a transforming growth factor-β- and RA-dependent manner in vitro. In the ManLNs, migratory CD103-CD11b+ cDCs also showed RA-producing activity. After the sublingual application of fluorescent OVA, fluorescence was detected in oral macrophages in tissues, followed by migratory CD103-CD11b+ cDCs in ManLNs and migratory CD103-CD11b+ cDCs were the main APCs responsible for the induction of sublingual antigen-specific Treg cells. The transfer of OVA-SLIT-induced Treg cells suppressed the OVA-induced hypersensitivity response. These results suggest that oral CD103-CD11b+ cDCs transport sublingual antigens to draining ManLNs and induce antigen-specific Foxp3+ Treg cells, and, thus, provide a rationale for developing cDC-based therapeutic approaches in SLIT.
巻・号 10(1)
ページ 79-90
公開日 2017-1-1
DOI 10.1038/mi.2016.46
PII mi201646
PMID 27166558
MeSH Animals Antigen Presentation Antigens / immunology Antigens, CD / metabolism CD11b Antigen / metabolism Cell Differentiation Cells, Cultured Dendritic Cells / immunology* Dendritic Cells / transplantation Disease Models, Animal Forkhead Transcription Factors / metabolism Humans Hypersensitivity / immunology Hypersensitivity / therapy* Integrin alpha Chains / metabolism Lymph Nodes / immunology* Lymphocyte Activation Mice Mice, Inbred C57BL Mice, Transgenic Ovalbumin / immunology Sublingual Immunotherapy / methods* T-Lymphocytes, Regulatory / immunology*
IF 6.726
引用数 19
WOS 分野 IMMUNOLOGY
リソース情報
実験動物マウス RBRC00144