RRC ID 36840
Author Tran ML, Roberts AW.
Title Cellulose synthase gene expression profiling of Physcomitrella patens.
Journal Plant Biol (Stuttg)
Abstract The cellulose synthase (CESA) gene family of seed plants comprises six clades that encode isoforms with conserved expression patterns and distinct functions in cellulose synthesis complex (CSC) formation and primary and secondary cell wall synthesis. In mosses, which have rosette CSCs like those of seed plants but lack lignified secondary cell walls, the CESA gene family diversified independently and includes no members of the six functionally distinct seed plant clades. There are seven CESA isoforms encoded in the genome of the moss Physcomitrella patens. However, only PpCESA5 has been characterised functionally, and little information is available on the expression of other PpCESA family members. We have profiled PpCESA expression through quantitative RT-PCR, analysis of promoter-reporter lines, and cluster analysis of public microarray data in an effort to identify expression and co-expression patterns that could help reveal the functions of PpCESA isoforms in protein complex formation and development of specific tissues. In contrast to the tissue-specific expression observed for seed plant CESAs, each of the PpCESAs was broadly expressed throughout most developing tissues. Although a few statistically significant differences in expression of PpCESAs were noted when some tissues and hormone treatments were compared, no strong co-expression patterns were observed. Along with CESA phylogenies and lack of single PpCESA mutant phenotypes reported elsewhere, broad overlapping expression of the PpCESAs indicates a high degree of inter-changeability and is consistent with a different pattern of functional specialisation in the evolution of the seed plant and moss CESA families.
Volume 18(3)
Pages 362-8
Published 2016-5
DOI 10.1111/plb.12416
PMID 26572930
MeSH Biological Evolution Bryopsida / cytology Bryopsida / enzymology* Bryopsida / genetics Cluster Analysis Gene Expression Profiling Gene Expression Regulation, Plant Genes, Reporter Glucosyltransferases / genetics* Isoenzymes Plant Proteins / genetics
IF 2.156
Times Cited 0
Arabidopsis / Cultured plant cells, genes pdp24095