Reference - Detail
| RRC ID | 36855 |
|---|---|
| Author | Saida S, Watanabe K, Kato I, Fujino H, Umeda K, Okamoto S, Uemoto S, Hishiki T, Yoshida H, Tanaka S, Adachi S, Niwa A, Nakahata T, Heike T. |
| Title | Prognostic significance of aminopeptidase-N (CD13) in hepatoblastoma. |
| Journal | Pediatr Int |
| Abstract |
BACKGROUND:Hepatoblastoma is a rare childhood malignant tumor that originates from immature hepatic cells. Aminopeptidase-N(CD13), an ectopeptidase that promotes tumor invasion and metastasis, is expressed in fetal stage hepatic progenitor cells, although its role in hepatoblastoma remains unclear. METHODS:The expression pattern of CD13 was investigated on immunohistochemistry in 30 tissue samples from 27 hepatoblastoma patients (16 with predominantly embryonal [pE] histology and 14 with predominantly fetal [pF] histology). Immunoreactive score (IRS) was used to quantify staining data, and the relationship between CD13 expression, clinicopathological factors, and clinical outcome was investigated. The biological function of CD13 was also examined in the hepatoblastoma cell lines Huh6 and HepG2. RESULTS:All specimens stained positive for CD13, with higher CD13 expression in pE than in pF hepatoblastoma samples (median IRS, 4; range, 2-9 vs 2; range, 1-4). Strong CD13 expression was correlated with vascular invasion. Five year event-free survival and overall survival were better in patients with CD13(low) than in those with CD13(high) tumors (100% vs 51.0%, P = 0.026; and 100% vs 74.0%, P = 0.114, respectively). A CD13-neutralizing antibody and the potent CD13 inhibitor, Ubenimex, suppressed invasive activity in HepG2 cells in vitro. CONCLUSIONS:CD13 expression is associated with hepatoblastoma invasiveness and could be a novel prognostic marker for hepatoblastoma. |
| Volume | 57(4) |
| Pages | 558-66 |
| Published | 2015-8-1 |
| DOI | 10.1111/ped.12597 |
| PMID | 25682862 |
| MeSH | Biopsy CD13 Antigens / biosynthesis CD13 Antigens / genetics* Cell Line, Tumor Child Child, Preschool DNA, Neoplasm / genetics* Female Gene Expression Regulation, Neoplastic* Hepatoblastoma / diagnosis Hepatoblastoma / enzymology Hepatoblastoma / genetics* Humans Immunohistochemistry Infant Infant, Newborn Liver / enzymology Liver / pathology* Liver Neoplasms / enzymology Liver Neoplasms / genetics* Liver Neoplasms / pathology Male Neoplasm Staging Prognosis Reverse Transcriptase Polymerase Chain Reaction |
| IF | 1.139 |
| Times Cited | 3 |
| WOS Category | PEDIATRICS |
| Resource | |
| Human and Animal Cells | HuH-6(RCB1367) Hep G2 |