RRC ID |
36858
|
著者 |
Li TC, Iwasaki K, Katano H, Kataoka M, Nagata N, Kobayashi K, Mizutani T, Takeda N, Wakita T, Suzuki T.
|
タイトル |
Characterization of self-assembled virus-like particles of Merkel cell polyomavirus.
|
ジャーナル |
PLoS One
|
Abstract |
In our recombinant baculovirus system, VP1 protein of merkel cell polyomavirus (MCPyV), which is implicated as a causative agent in Merkel cell carcinoma, was self-assembled into MCPyV-like particles (MCPyV-LP) with two different sizes in insect cells, followed by being released into the culture medium. DNA molecules of 1.5- to 5-kb, which were derived from host insect cells, were packaged in large, ~50-nm spherical particles but not in small, ~25-nm particles. Structure reconstruction using cryo-electron microscopy showed that large MCPyV-LPs are composed of 72 pentameric capsomeres arranged in a T = 7 icosahedral surface lattice and are 48 nm in diameter. The MCPyV-LPs did not share antigenic determinants with BK- and JC viruses (BKPyV and JCPyV). The VLP-based enzyme immunoassay was applied to investigate age-specific prevalence of MCPyV infection in the general Japanese population aged 1-70 years. While seroprevalence of MCPyV increased with age in children and young individuals, its seropositivity in each age group was lower compared with BKPyV and JCPyV.
|
巻・号 |
10(2)
|
ページ |
e0115646
|
公開日 |
2015-1-1
|
DOI |
10.1371/journal.pone.0115646
|
PII |
PONE-D-14-39015
|
PMID |
25671590
|
PMC |
PMC4324643
|
MeSH |
Adolescent
Adult
Aged
Animals
Baculoviridae / genetics
Capsid Proteins / genetics
Capsid Proteins / isolation & purification
Capsid Proteins / metabolism*
Cell Line
Child
Child, Preschool
Gene Expression
Humans
Infant
Insecta
JC Virus / immunology
Japan
Merkel cell polyomavirus* / genetics
Merkel cell polyomavirus* / metabolism
Middle Aged
Polyomavirus Infections / immunology
Young Adult
|
IF |
2.74
|
引用数 |
8
|
WOS 分野
|
VIROLOGY
|
リソース情報 |
ヒト・動物細胞 |
Sf9(RCB0563) |