RRC ID 36858
著者 Li TC, Iwasaki K, Katano H, Kataoka M, Nagata N, Kobayashi K, Mizutani T, Takeda N, Wakita T, Suzuki T.
タイトル Characterization of self-assembled virus-like particles of Merkel cell polyomavirus.
ジャーナル PLoS One
Abstract In our recombinant baculovirus system, VP1 protein of merkel cell polyomavirus (MCPyV), which is implicated as a causative agent in Merkel cell carcinoma, was self-assembled into MCPyV-like particles (MCPyV-LP) with two different sizes in insect cells, followed by being released into the culture medium. DNA molecules of 1.5- to 5-kb, which were derived from host insect cells, were packaged in large, ~50-nm spherical particles but not in small, ~25-nm particles. Structure reconstruction using cryo-electron microscopy showed that large MCPyV-LPs are composed of 72 pentameric capsomeres arranged in a T = 7 icosahedral surface lattice and are 48 nm in diameter. The MCPyV-LPs did not share antigenic determinants with BK- and JC viruses (BKPyV and JCPyV). The VLP-based enzyme immunoassay was applied to investigate age-specific prevalence of MCPyV infection in the general Japanese population aged 1-70 years. While seroprevalence of MCPyV increased with age in children and young individuals, its seropositivity in each age group was lower compared with BKPyV and JCPyV.
巻・号 10(2)
ページ e0115646
公開日 2015-1-1
DOI 10.1371/journal.pone.0115646
PII PONE-D-14-39015
PMID 25671590
PMC PMC4324643
MeSH Adolescent Adult Aged Animals Baculoviridae / genetics Capsid Proteins / genetics Capsid Proteins / isolation & purification Capsid Proteins / metabolism* Cell Line Child Child, Preschool Gene Expression Humans Infant Insecta JC Virus / immunology Japan Merkel cell polyomavirus* / genetics Merkel cell polyomavirus* / metabolism Middle Aged Polyomavirus Infections / immunology Young Adult
IF 2.74
引用数 8
WOS 分野 VIROLOGY
リソース情報
ヒト・動物細胞 Sf9(RCB0563)