論文 - 詳細
| RRC ID | 36869 |
|---|---|
| 著者 | He J, Miyazaki J, Wang N, Tsurui H, Kobayashi T. |
| タイトル | Label-free imaging of melanoma with nonlinear photothermal microscopy. |
| ジャーナル | Opt Lett |
| Abstract |
Nonlinear photothermal microscopy, in which the intensity of the pump heating beam is modulated at f and the photothermal signal is extracted from the probe beam with a lock-in amplifier referred to 2f, is applied to the imaging of mouse melanoma without any staining. The pump and probe pulses, with central wavelengths of 488 and 632 nm, and a pulse duration of ∼100 ps, are filtered from a compact commercial supercontinuum fiber laser source. An auto-balanced detector is applied to accumulate the signal and remove the laser noise of the probe. The spatial resolution of the nonlinear photothermal imaging is enhanced by ∼18% in both theoretical calculations and experiments, compared with a linear photothermal mechanism, and the resolution enhancement is theoretically ∼42% compared with conventional optical microscopy. This imaging technique shows possibilities for the clinical evaluation of melanoma with a high contrast and spatial resolution. |
| 巻・号 | 40(7) |
| ページ | 1141-4 |
| 公開日 | 2015-4-1 |
| DOI | 10.1364/OL.40.001141 |
| PII | 312932 |
| PMID | 25831277 |
| MeSH | Animals Hot Temperature* Melanoma / pathology* Mice Microscopy / methods* Molecular Imaging / methods* |
| IF | 3.714 |
| 引用数 | 12 |
| WOS 分野 | OPTICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16 melanoma(RCB1283) |