| RRC ID |
36872
|
| Author |
Suzuki Y, Nakagawa K, Kato S, Tatewaki N, Mizuochi S, Ito J, Eitsuka T, Nishida H, Miyazawa T.
|
| Title |
Metabolism and cytotoxic effects of phosphatidylcholine hydroperoxide in human hepatoma HepG2 cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
In this study, we investigated cellular uptake and metabolism of phosphatidylcholine hydroperoxide (PCOOH) in human hepatoma HepG2 cells by high performance liquid chromatography-tandem mass spectrometry, and then evaluated whether PCOOH or its metabolites cause pathophysiological effects such as cytotoxicity and apoptosis. Although we found that most PCOOH was reduced to PC hydroxide in HepG2 cells, the remaining PCOOH caused cytotoxic effects that may be mediated through an unusual apoptosis pathway. These results will enhance our fundamental understanding of how PCOOH, which is present in oxidized low density lipoproteins, is involved in the development of atherosclerosis.
|
| Volume |
458(4)
|
| Pages |
920-7
|
| Published |
2015-3-20
|
| DOI |
10.1016/j.bbrc.2015.02.063
|
| PII |
S0006-291X(15)00291-0
|
| PMID |
25704087
|
| MeSH |
Apoptosis*
Cell Cycle
Hep G2 Cells / cytology*
Hep G2 Cells / metabolism
Hep G2 Cells / pathology
Humans
Membrane Potential, Mitochondrial
Phosphatidylcholines / metabolism*
Phosphatidylcholines / toxicity
|
| IF |
2.985
|
| Times Cited |
3
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1886) |
