論文 - 詳細
| RRC ID | 36876 |
|---|---|
| 著者 | Yanagida A, Nakauchi H, Kamiya A. |
| タイトル | Generation and In Vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Stem cells have the unique properties of self-renewal and multipotency (producing progeny belonging to two or more lineages). Induced pluripotent stem (iPS) cells can be generated from somatic cells by simultaneous expression of pluripotent factors (Oct3/4, Klf4, Sox2, and c-Myc). They share the same properties as embryonic stem (ES) cells and can differentiate into several tissue cells, i.e., neurons, hematopoietic cells, and liver cells. Therefore, iPS cells are suitable candidate cells for regenerative medicine and analyses of disease mechanisms.The liver is the major organ that regulates a multitude of metabolic functions. Hepatocytes are the major cell type populating the liver parenchyma and express several metabolic enzymes that are necessary for liver functions. Although hepatocytes are essential for maintaining homeostasis, it is difficult to alter artificial and transplanted cells because of their multifunctionality, donor shortage, and immunorejection risk. During liver development, hepatic progenitor cells in the fetal liver differentiate into both mature hepatocytes and cholangiocytes. As hepatic progenitor cells have bipotency and high proliferation ability, they could present a potential source for generating transplantable cells or as a liver study model. Here we describe the induction and purification of hepatic progenitor cells derived from human iPS cells. These cells can proliferate for a long term under suitable culture conditions. |
| 巻・号 | 1357 |
| ページ | 295-310 |
| 公開日 | 2016-1-1 |
| DOI | 10.1007/7651_2015_199 |
| PMID | 25697415 |
| MeSH | Animals Cell Culture Techniques / methods* Cell Differentiation Cell Lineage Cells, Cultured Cellular Reprogramming Fibroblasts / cytology Flow Cytometry Gene Expression Regulation / drug effects Hepatocytes / cytology* Humans Induced Pluripotent Stem Cells / cytology* Kruppel-Like Factor 4 Mice Mice, Inbred ICR Mitomycin / pharmacology Skin / cytology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1886) |