RRC ID 36899
Author Ojima T, Shibata E, Saito S, Toyoda M, Nakajima H, Yamazaki-Inoue M, Miyagawa Y, Kiyokawa N, Fujimoto JI, Sato T, Umezawa A.
Title Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells.
Journal Sci Rep
Abstract Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells' genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells.
Volume 5
Pages 14988
Published 2015-10-19
DOI 10.1038/srep14988
PII srep14988
PMID 26477663
PMC PMC4609952
MeSH ABO Blood-Group System / genetics Biomarkers Cell Differentiation* Cell Line Chromatography, Liquid Embryoid Bodies / cytology Embryoid Bodies / metabolism Flow Cytometry Genetic Variation Genotype Glycolipids / metabolism* Glycosphingolipids / metabolism Humans Immunohistochemistry Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism* Neural Stem Cells / cytology Neural Stem Cells / metabolism Polysaccharides / biosynthesis Tandem Mass Spectrometry
IF 3.998
Times Cited 14
DNA material pENTR/A4GALT (RDB14006) pcDNA3/B3GNT5 (RDB14008) pGEM-T/ST3GAL5 (RDB14007) pENTR/B4GALNT1 (RDB14009) pENTR/B3GALT5 (RDB14010) pENTR/FUT1 (RDB14011) pENTR/FUT2 (RDB14012).