論文 - 詳細
| RRC ID | 36899 |
|---|---|
| 著者 | Ojima T, Shibata E, Saito S, Toyoda M, Nakajima H, Yamazaki-Inoue M, Miyagawa Y, Kiyokawa N, Fujimoto JI, Sato T, Umezawa A. |
| タイトル | Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells. |
| ジャーナル | Sci Rep |
| Abstract |
Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells' genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells. |
| 巻・号 | 5 |
| ページ | 14988 |
| 公開日 | 2015-10-19 |
| DOI | 10.1038/srep14988 |
| PII | srep14988 |
| PMID | 26477663 |
| PMC | PMC4609952 |
| MeSH | ABO Blood-Group System / genetics Biomarkers Cell Differentiation* Cell Line Chromatography, Liquid Embryoid Bodies / cytology Embryoid Bodies / metabolism Flow Cytometry Genetic Variation Genotype Glycolipids / metabolism* Glycosphingolipids / metabolism Humans Immunohistochemistry Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism* Neural Stem Cells / cytology Neural Stem Cells / metabolism Polysaccharides / biosynthesis Tandem Mass Spectrometry |
| IF | 3.998 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pENTR/A4GALT (RDB14006) pcDNA3/B3GNT5 (RDB14008) pGEM-T/ST3GAL5 (RDB14007) pENTR/B4GALNT1 (RDB14009) pENTR/B3GALT5 (RDB14010) pENTR/FUT1 (RDB14011) pENTR/FUT2 (RDB14012). |