| RRC ID |
36911
|
| Author |
Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C.
|
| Title |
Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin.
|
| Journal |
J Biol Chem
|
| Abstract |
As acidic glycocalyx on primary mouse microglial cells and a mouse microglial cell line Ra2, expression of polysialic acid (polySia/PSA), a polymer of the sialic acid Neu5Ac (N-acetylneuraminic acid), was demonstrated. PolySia is known to modulate cell adhesion, migration, and localization of neurotrophins mainly on neural cells. PolySia on Ra2 cells disappeared very rapidly after an inflammatory stimulus. Results of knockdown and inhibitor studies indicated that rapid surface clearance of polySia was achieved by secretion of endogenous sialidase Neu1 as an exovesicular component. Neu1-mediated polySia turnover was accompanied by the release of brain-derived neurotrophic factor normally retained by polySia molecules. Introduction of a single oxygen atom change into polySia by exogenous feeding of the non-neural sialic acid Neu5Gc (N-glycolylneuraminic acid) caused resistance to Neu1-induced polySia turnover and also inhibited the associated release of brain-derived neurotrophic factor. These results indicate the importance of rapid turnover of the polySia glycocalyx by exovesicular sialidases in neurotrophin regulation.
|
| Volume |
290(21)
|
| Pages |
13202-14
|
| Published |
2015-5-22
|
| DOI |
10.1074/jbc.M115.638759
|
| PII |
S0021-9258(20)33684-X
|
| PMID |
25750127
|
| PMC |
PMC4505574
|
| MeSH |
Animals
Animals, Newborn
Blotting, Western
Brain-Derived Neurotrophic Factor / metabolism
Cell Membrane / metabolism*
Cells, Cultured
Extracellular Matrix / enzymology*
Fluorescent Antibody Technique
Glycocalyx / metabolism*
Membrane Microdomains / metabolism
Mice
Mice, Inbred C57BL
Microglia / cytology
Microglia / metabolism*
Nerve Growth Factors / genetics
Nerve Growth Factors / metabolism*
Neuraminidase / genetics
Neuraminidase / metabolism*
Oxygen / metabolism
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Sialic Acids / metabolism*
|
| IF |
4.238
|
| Times Cited |
44
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
COS-7(RCB0539) |
