RRC ID 36911
著者 Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C.
タイトル Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin.
ジャーナル J Biol Chem
Abstract As acidic glycocalyx on primary mouse microglial cells and a mouse microglial cell line Ra2, expression of polysialic acid (polySia/PSA), a polymer of the sialic acid Neu5Ac (N-acetylneuraminic acid), was demonstrated. PolySia is known to modulate cell adhesion, migration, and localization of neurotrophins mainly on neural cells. PolySia on Ra2 cells disappeared very rapidly after an inflammatory stimulus. Results of knockdown and inhibitor studies indicated that rapid surface clearance of polySia was achieved by secretion of endogenous sialidase Neu1 as an exovesicular component. Neu1-mediated polySia turnover was accompanied by the release of brain-derived neurotrophic factor normally retained by polySia molecules. Introduction of a single oxygen atom change into polySia by exogenous feeding of the non-neural sialic acid Neu5Gc (N-glycolylneuraminic acid) caused resistance to Neu1-induced polySia turnover and also inhibited the associated release of brain-derived neurotrophic factor. These results indicate the importance of rapid turnover of the polySia glycocalyx by exovesicular sialidases in neurotrophin regulation.
巻・号 290(21)
ページ 13202-14
公開日 2015-5-22
DOI 10.1074/jbc.M115.638759
PII S0021-9258(20)33684-X
PMID 25750127
PMC PMC4505574
MeSH Animals Animals, Newborn Blotting, Western Brain-Derived Neurotrophic Factor / metabolism Cell Membrane / metabolism* Cells, Cultured Extracellular Matrix / enzymology* Fluorescent Antibody Technique Glycocalyx / metabolism* Membrane Microdomains / metabolism Mice Mice, Inbred C57BL Microglia / cytology Microglia / metabolism* Nerve Growth Factors / genetics Nerve Growth Factors / metabolism* Neuraminidase / genetics Neuraminidase / metabolism* Oxygen / metabolism RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sialic Acids / metabolism*
IF 4.238
引用数 44
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 COS-7(RCB0539)