論文 - 詳細
| RRC ID | 36915 |
|---|---|
| 著者 | Kuramitsu-Fujimoto S, Ariyoshi W, Saito N, Okinaga T, Kamo M, Ishisaki A, Takata T, Yamaguchi K, Nishihara T. |
| タイトル | Novel biological activity of ameloblastin in enamel matrix derivative. |
| ジャーナル | J Appl Oral Sci |
| Abstract |
OBJECTIVE:Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration. However, the effects of EMD on gingival epithelial cells during regeneration of periodontal tissues are unclear. In this in vitro study, we purified ameloblastin from EMD and investigated its biological effects on epithelial cells. MATERIAL AND METHODS:Bioactive fractions were purified from EMD by reversed-phase high-performance liquid chromatography using hydrophobic support with a C18 column. The mouse gingival epithelial cell line GE-1 and human oral squamous cell carcinoma line SCC-25 were treated with purified EMD fraction, and cell survival was assessed with a WST-1 assay. To identify the proteins in bioactive fractions of EMD, we used proteome analysis with two-dimensional gel electrophoresis followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. RESULTS:Purified fractions from EMD suppressed proliferation of GE-1 and SCC-25. LC-MS/MS revealed that ameloblastin in EMD is the component responsible for inhibiting epithelial cell proliferation. The inhibitory effect of ameloblastin on the proliferation of GE-1 and SCC-25 was confirmed using recombinant protein. CONCLUSION:The inhibitory effects of EMD on epithelial cell proliferation are caused by the biological activities of ameloblastin, which suggests that ameloblastin is involved in regulating epithelial downgrowth in periodontal tissues. |
| 巻・号 | 23(1) |
| ページ | 49-55 |
| 公開日 | 2015-1-1 |
| DOI | 10.1590/1678-775720140291 |
| PII | S1678-77572015000100049 |
| PMID | 25760267 |
| PMC | PMC4349119 |
| MeSH | Animals Cell Proliferation / drug effects Cell Survival / drug effects Cells, Cultured Chromatography, High Pressure Liquid Dental Enamel Proteins / pharmacology* Electrophoresis, Gel, Two-Dimensional Epithelial Cells / cytology Epithelial Cells / drug effects* Gingiva / cytology Gingiva / drug effects Guided Tissue Regeneration, Periodontal / methods Humans Mice Periodontitis / drug therapy Periodontium / drug effects* Reference Values Reproducibility of Results Silver Staining Time Factors |
| IF | 1.797 |
| 引用数 | 4 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | GE1(RCB1709) |