論文 - 詳細
| RRC ID | 36931 |
|---|---|
| 著者 | Kiyomiya H, Ariyoshi W, Okinaga T, Kaneuji T, Mitsugi S, Sakurai T, Habu M, Yoshioka I, Tominaga K, Nishihara T. |
| タイトル | IL-33 inhibits RANKL-induced osteoclast formation through the regulation of Blimp-1 and IRF-8 expression. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Interleukin (IL)-33 is a recently discovered proinflammatory cytokine that belongs to the IL-1 family. Several studies have reported that IL-33 inhibits osteoclast differentiation. However, the mechanism of IL-33 regulation of osteoclastogenesis remains unclear. In the present study, we examined the effect of IL-33 on osteoclast formation in vitro. IL-33 suppressed osteoclast formation in both mouse bone marrow cells and monocyte/macrophage cell line RAW264.7 cells induced by receptor activator of NF-κB ligand (RANKL) and/or macrophage stimulating factor (M-CSF). IL-33 also inhibited the expression of RANKL-induced nuclear factor of activated T-cell cytoplasmic 1 (NFATc1), thereby decreasing the expression of osteoclastogenesis-related marker genes, including Cathepsin K, Osteoclast stimulatory transmembrane protein (Oc-stamp) and Tartrate-resistant acid phosphatase (Trap). Blockage of IL-33-ST2 binding suppressed the IL-33-mediated inhibition of NFATc1. RANKL-induced B-lymphocyte-induced maturation protein-1 (Blimp-1) expression was also suppressed by IL-33, which was followed by the stimulation of anti-osteoclastic genes such as interferon regulatory factor-8 (IRF-8). These results suggest that IL-33-ST2 interactions down-regulate both RANKL-induced NFATc1 activation and osteoclast differentiation via the regulation of Blimp-1 and IRF-8 expression. |
| 巻・号 | 460(2) |
| ページ | 320-6 |
| 公開日 | 2015-5-1 |
| DOI | 10.1016/j.bbrc.2015.03.033 |
| PII | S0006-291X(15)00468-4 |
| PMID | 25795135 |
| MeSH | Animals Base Sequence Cell Line Cells, Cultured DNA Primers Female Interferon Regulatory Factors / genetics Interferon Regulatory Factors / metabolism* Interleukin-33 Interleukins / physiology* Male Mice NFATC Transcription Factors / genetics NFATC Transcription Factors / metabolism Osteoclasts / cytology* Positive Regulatory Domain I-Binding Factor 1 RANK Ligand / antagonists & inhibitors* RANK Ligand / physiology Real-Time Polymerase Chain Reaction Signal Transduction Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 2.985 |
| 引用数 | 18 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |