| RRC ID |
36931
|
| 著者 |
Kiyomiya H, Ariyoshi W, Okinaga T, Kaneuji T, Mitsugi S, Sakurai T, Habu M, Yoshioka I, Tominaga K, Nishihara T.
|
| タイトル |
IL-33 inhibits RANKL-induced osteoclast formation through the regulation of Blimp-1 and IRF-8 expression.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Interleukin (IL)-33 is a recently discovered proinflammatory cytokine that belongs to the IL-1 family. Several studies have reported that IL-33 inhibits osteoclast differentiation. However, the mechanism of IL-33 regulation of osteoclastogenesis remains unclear. In the present study, we examined the effect of IL-33 on osteoclast formation in vitro. IL-33 suppressed osteoclast formation in both mouse bone marrow cells and monocyte/macrophage cell line RAW264.7 cells induced by receptor activator of NF-κB ligand (RANKL) and/or macrophage stimulating factor (M-CSF). IL-33 also inhibited the expression of RANKL-induced nuclear factor of activated T-cell cytoplasmic 1 (NFATc1), thereby decreasing the expression of osteoclastogenesis-related marker genes, including Cathepsin K, Osteoclast stimulatory transmembrane protein (Oc-stamp) and Tartrate-resistant acid phosphatase (Trap). Blockage of IL-33-ST2 binding suppressed the IL-33-mediated inhibition of NFATc1. RANKL-induced B-lymphocyte-induced maturation protein-1 (Blimp-1) expression was also suppressed by IL-33, which was followed by the stimulation of anti-osteoclastic genes such as interferon regulatory factor-8 (IRF-8). These results suggest that IL-33-ST2 interactions down-regulate both RANKL-induced NFATc1 activation and osteoclast differentiation via the regulation of Blimp-1 and IRF-8 expression.
|
| 巻・号 |
460(2)
|
| ページ |
320-6
|
| 公開日 |
2015-5-1
|
| DOI |
10.1016/j.bbrc.2015.03.033
|
| PII |
S0006-291X(15)00468-4
|
| PMID |
25795135
|
| MeSH |
Animals
Base Sequence
Cell Line
Cells, Cultured
DNA Primers
Female
Interferon Regulatory Factors / genetics
Interferon Regulatory Factors / metabolism*
Interleukin-33
Interleukins / physiology*
Male
Mice
NFATC Transcription Factors / genetics
NFATC Transcription Factors / metabolism
Osteoclasts / cytology*
Positive Regulatory Domain I-Binding Factor 1
RANK Ligand / antagonists & inhibitors*
RANK Ligand / physiology
Real-Time Polymerase Chain Reaction
Signal Transduction
Transcription Factors / genetics
Transcription Factors / metabolism*
|
| IF |
2.985
|
| 引用数 |
18
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
