論文 - 詳細
| RRC ID | 36934 |
|---|---|
| 著者 | Mira A, Yamashita S, Katakura Y, Shimizu K. |
| タイトル | In vitro neuroprotective activities of compounds from Angelica shikokiana Makino. |
| ジャーナル | Molecules |
| Abstract |
Angelica shikokiana is widely marketed in Japan as a dietary food supplement. With a focus on neurodegenerative conditions such as Alzheimer's disease, the aerial part was extracted and through bio-guided fractionation, fifteen compounds [α-glutinol, β-amyrin, kaempferol, luteolin, quercetin, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, methyl chlorogenate, chlorogenic acid, hyuganin E, 5-(hydroxymethyl)-2-furaldehyde, β-sitosterol-3-O-glucoside, adenosine (isolated for the first time from A. shikokiana), isoepoxypteryxin and isopteryxin] were isolated. Isolated compounds were evaluated for in vitro neuroprotection using acetylcholine esterase inhibitory, protection against hydrogen peroxide and amyloid β peptide (Aβ25-35)-induced neurotoxicity in neuro-2A cells, scavenging of hydroxyl radicals and intracellular reactive oxygen species and thioflavin T assays. Quercetin showed the strongest AChE inhibition (IC50 value = 35.5 µM) through binding to His-440 and Tyr-70 residues at the catalytic and anionic sites of acetylcholine esterase, respectively. Chlorogenic acid, its methyl ester, quercetin and luteolin could significantly protect neuro-2A cells against H2O2-induced neurotoxicity and scavenge hydroxyl radical and intracellular reactive oxygen species. Kaempferol-3-O-rutinoiside, hyuganin E and isoepoxypteryxin significantly decreased Aβ25-35-induced neurotoxicity and Th-T fluorescence. To the best of our knowledge, this is the first report about neuroprotection of hyuganin E and isoepoxypteryxin against Aβ25-35-induced neurotoxicity. |
| 巻・号 | 20(3) |
| ページ | 4813-32 |
| 公開日 | 2015-3-16 |
| DOI | 10.3390/molecules20034813 |
| PII | molecules20034813 |
| PMID | 25786165 |
| PMC | PMC6272295 |
| MeSH | Amyloid beta-Peptides / toxicity Angelica / chemistry* Animals Brain Neoplasms / drug therapy* Cell Line, Tumor Hydrogen Peroxide / toxicity In Vitro Techniques Japan Mice Neuroblastoma / drug therapy* Neuroprotective Agents / chemistry Neuroprotective Agents / isolation & purification* Neuroprotective Agents / pharmacology* Peptide Fragments / toxicity Plant Components, Aerial / chemistry Plant Extracts / chemistry* Plant Extracts / pharmacology* Quercetin / isolation & purification Quercetin / pharmacology Reactive Oxygen Species / metabolism |
| IF | 3.267 |
| 引用数 | 10 |
| WOS 分野 | CHEMISTRY, ORGANIC |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | NB2a(RCB2639) |