RRC ID 36934
Author Mira A, Yamashita S, Katakura Y, Shimizu K.
Title In vitro neuroprotective activities of compounds from Angelica shikokiana Makino.
Journal Molecules
Abstract Angelica shikokiana is widely marketed in Japan as a dietary food supplement. With a focus on neurodegenerative conditions such as Alzheimer's disease, the aerial part was extracted and through bio-guided fractionation, fifteen compounds [α-glutinol, β-amyrin, kaempferol, luteolin, quercetin, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, methyl chlorogenate, chlorogenic acid, hyuganin E, 5-(hydroxymethyl)-2-furaldehyde, β-sitosterol-3-O-glucoside, adenosine (isolated for the first time from A. shikokiana), isoepoxypteryxin and isopteryxin] were isolated. Isolated compounds were evaluated for in vitro neuroprotection using acetylcholine esterase inhibitory, protection against hydrogen peroxide and amyloid β peptide (Aβ25-35)-induced neurotoxicity in neuro-2A cells, scavenging of hydroxyl radicals and intracellular reactive oxygen species and thioflavin T assays. Quercetin showed the strongest AChE inhibition (IC50 value = 35.5 µM) through binding to His-440 and Tyr-70 residues at the catalytic and anionic sites of acetylcholine esterase, respectively. Chlorogenic acid, its methyl ester, quercetin and luteolin could significantly protect neuro-2A cells against H2O2-induced neurotoxicity and scavenge hydroxyl radical and intracellular reactive oxygen species. Kaempferol-3-O-rutinoiside, hyuganin E and isoepoxypteryxin significantly decreased Aβ25-35-induced neurotoxicity and Th-T fluorescence. To the best of our knowledge, this is the first report about neuroprotection of hyuganin E and isoepoxypteryxin against Aβ25-35-induced neurotoxicity.
Volume 20(3)
Pages 4813-32
Published 2015-3-16
DOI 10.3390/molecules20034813
PII molecules20034813
PMID 25786165
PMC PMC6272295
MeSH Amyloid beta-Peptides / toxicity Angelica / chemistry* Animals Brain Neoplasms / drug therapy* Cell Line, Tumor Hydrogen Peroxide / toxicity In Vitro Techniques Japan Mice Neuroblastoma / drug therapy* Neuroprotective Agents / chemistry Neuroprotective Agents / isolation & purification* Neuroprotective Agents / pharmacology* Peptide Fragments / toxicity Plant Components, Aerial / chemistry Plant Extracts / chemistry* Plant Extracts / pharmacology* Quercetin / isolation & purification Quercetin / pharmacology Reactive Oxygen Species / metabolism
IF 3.06
Times Cited 10
WOS Category CHEMISTRY, ORGANIC
Resource
Human and Animal Cells