RRC ID 36951
著者 Cueno ME, Kamio N, Seki K, Kurita-Ochiai T, Ochiai K.
タイトル High butyric acid amounts induce oxidative stress, alter calcium homeostasis, and cause neurite retraction in nerve growth factor-treated PC12 cells.
ジャーナル Cell Stress Chaperones
Abstract Butyric acid (BA) is a common secondary metabolite by-product produced by oral pathogenic bacteria and is detected in high amounts in the gingival tissue of patients with periodontal disease. Previous works have demonstrated that BA can cause oxidative stress in various cell types; however, this was never explored using neuronal cells. Here, we exposed nerve growth factor (NGF)-treated PC1(2) cells to varying BA concentrations (0.5, 1.0, 5.0 mM). We measured total heme, H(2)O(2), catalase, and calcium levels through biochemical assays and visualized the neurite outgrowth after BA treatment. Similarly, we determined the effects of other common periodontal short-chain fatty acids (SCFAs) on neurite outgrowth for comparison. We found that high (1.0 and 5.0 mM) BA concentrations induced oxidative stress and altered calcium homeostasis, whereas low (0.5 mM) BA concentration had no significant effect. Moreover, compared to other SCFAs, we established that only BA was able to induce neurite retraction.
巻・号 20(4)
ページ 709-13
公開日 2015-7-1
DOI 10.1007/s12192-015-0584-1
PMID 25808460
PMC PMC4463921
MeSH Animals Butyric Acid / toxicity* Calcium / metabolism* Catalase / metabolism Fatty Acids / pharmacology Heme / metabolism Hydrogen Peroxide / metabolism Nerve Growth Factor / pharmacology* Neurites / drug effects Neurites / metabolism Oxidative Stress / drug effects* PC12 Cells Rats
IF 2.892
引用数 11
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 PC-12(RCB0009)