Reference - Detail
|Author||Haitani Y, Takagi H.|
|Title||Rsp5 is required for the nuclear export of mRNA of HSF1 and MSN2/4 under stress conditions in Saccharomyces cerevisiae.|
Rsp5 is an essential and multi-functional E3 ubiquitin ligase in Saccharomyces cerevisiae. We previously isolated the Ala401Glu rsp5 mutant that is hypersensitive to various stresses. In rsp5(A401E) cells, the transcription of the stress protein genes was defective. To understand the mechanism by which Rsp5 regulates the expression of stress proteins, we analyzed the expression and localization of two major transcription factors, Hsf1 and Msn2/4, required for stress protein gene expression in S. cerevisiae. The mRNA levels of HSF1 and MSN2/4 in rsp5(A401E) cells were slightly lower than those of wild-type cells. An interesting finding is that the protein levels of HSF1 and Msn2/4 were remarkably defective in rsp5(A401E) cells after exposure to temperature up-shift and ethanol, although these proteins are mainly localized in the nucleus under these stress conditions. We also showed that the mRNAs of HSF1 and MSN2/4 were accumulated in the nucleus of rsp5(A401E) cells after exposure to temperature up-shift and ethanol, and even under non-stress conditions, suggesting that Rsp5 is required for the nuclear export of these mRNAs. These results indicate that, in response to environmental stresses, Rsp5 primarily regulates the expression of Hsf1 and Msn2/4 at the post-transcriptional level and is involved in the repair system of stress-induced abnormal proteins.
|MeSH||Active Transport, Cell Nucleus Base Sequence DNA, Fungal / genetics DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Endosomal Sorting Complexes Required for Transport Gene Expression Genes, Fungal Heat-Shock Proteins / genetics* Heat-Shock Proteins / metabolism In Situ Hybridization, Fluorescence Mutation Plasmids / genetics RNA Processing, Post-Transcriptional RNA, Fungal / genetics* RNA, Fungal / metabolism* RNA, Messenger / genetics* RNA, Messenger / metabolism* Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism* Transcription Factors / genetics* Transcription Factors / metabolism Transcription, Genetic Ubiquitin-Protein Ligase Complexes / genetics* Ubiquitin-Protein Ligase Complexes / metabolism*|
|WOS Category||GENETICS & HEREDITY CELL BIOLOGY|
|Yeast||pFA6a-GFP-kan BY25535 BY25536 BY25537 BY25538 BY25539 BY25540 BY25541 BYP6472 BYP6473 BYP6474|