| RRC ID |
36985
|
| 著者 |
Noguchi M, Kanari Y, Yokoya A, Narita A, Fujii K.
|
| タイトル |
Live-cell imaging study of mitochondrial morphology in mammalian cells exposed to X-rays.
|
| ジャーナル |
Radiat Prot Dosimetry
|
| Abstract |
Morphological changes in mitochondria induced by X-irradiation in normal murine mammary gland cells were studied with a live-cell microscopic imaging technique. Mitochondria were visualised by staining with a specific fluorescent probe in the cells, which express fluorescent ubiquitination-based cell-cycle indicator 2 (Fucci2) probes to visualise cell cycle. In unirradiated cells, the number of cells with fragmented mitochondria was about 20 % of the total cells through observation period (96 h). In irradiated cells, the population with fragmented mitochondria significantly increased depending on the absorbed dose. Particularly, for 8 Gy irradiation, the accumulation of fragmentation persists even in the cells whose cell cycle came to a stand (80 % in G1 (G0-like) phase). The fraction reached to a maximum at 96 h after irradiation. The kinetics of the fraction with fragmented mitochondria was similar to that for cells in S/G2/M phase (20 %) through the observation period (120 h). The evidences show that, in irradiated cells, some signals are continually released from a nucleus or cytoplasm even in the G0-like cells to operate some sort of protein machineries involved in mitochondrial fission. It is inferred that this delayed mitochondrial fragmentation is strongly related to their dysfunction, and hence might modulate radiobiological effects such as mutation or cell death.
|
| 巻・号 |
166(1-4)
|
| ページ |
101-3
|
| 公開日 |
2015-9-1
|
| DOI |
10.1093/rpd/ncv157
|
| PII |
ncv157
|
| PMID |
25883301
|
| MeSH |
Animals
Cell Cycle / radiation effects*
Cells, Cultured
Female
Fluorescent Dyes / analysis*
Image Processing, Computer-Assisted / methods*
Mammary Glands, Animal / radiation effects*
Mice
Mitochondria / physiology*
Mitochondria / radiation effects*
Mitosis / radiation effects*
Ubiquitination / radiation effects
X-Rays
|
| IF |
0.773
|
| 引用数 |
0
|
|
WOS 分野
|
ENVIRONMENTAL SCIENCES
NUCLEAR SCIENCE & TECHNOLOGY
PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
|
| リソース情報 |
| ヒト・動物細胞 |
NMuMG/Fucci2(RCB2868) |
