論文 - 詳細
| RRC ID | 36991 |
|---|---|
| 著者 | Shigeto J, Itoh Y, Hirao S, Ohira K, Fujita K, Tsutsumi Y. |
| タイトル | Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem. |
| ジャーナル | J Integr Plant Biol |
| Abstract |
Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-DNA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol-β-aryl (β-O-4) linkage type, especially β-O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants exhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved cell wall degradability. This study will contribute to progress in lignin bioengineering to improve lignocellulosic biomass. |
| 巻・号 | 57(4) |
| ページ | 349-56 |
| 公開日 | 2015-4-1 |
| DOI | 10.1111/jipb.12334 |
| PMID | 25644691 |
| MeSH | Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Wall / metabolism Cellulase / metabolism Cellulose / metabolism Gene Expression Regulation, Plant Gene Knockout Techniques Genes, Plant Glucose / metabolism Green Fluorescent Proteins / metabolism Lignin / metabolism* Mutation Peroxidases / genetics Peroxidases / metabolism* Plant Development Plant Stems / anatomy & histology* Plant Stems / metabolism* Protein Sorting Signals Protein Transport Subcellular Fractions / metabolism |
| IF | 4.885 |
| 引用数 | 37 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |