論文 - 詳細
| RRC ID | 36992 |
|---|---|
| 著者 | Shigeta T, Zaizen Y, Sugimoto Y, Nakamura Y, Matsuo T, Okamoto S. |
| タイトル | Heat shock protein 90 acts in brassinosteroid signaling through interaction with BES1/BZR1 transcription factor. |
| ジャーナル | J Plant Physiol |
| Abstract |
Brassinosteroids (BRs), a class of phytohormones, control various physiological and developmental processes in plants. Two highly homologous transcription factors, brassinosteroid insensitive 1-EMS-SUPRESSOR 1 (BES1) and brassinazole resistant 1 (BZR1), act downstream of BR signaling to control several thousands of putative target genes. We reported previously that BES1 forms a complex with a molecular chaperone: heat shock protein 90 (HSP90). This study demonstrates that the amino-terminal and central parts of BES1 are responsible for its physical interaction with HSP90.3 in vitro. Additionally, we present evidence that BZR1 is a novel HSP90 partner aside from two BR signaling components previously identified as its clients: BES1 and brassinosteroid insensitive 2 (BIN2). Furthermore, geldanamycin, an inhibitor of ATPase activity in HSP90, caused BES1 hyperphosphorylation and disrupted the expression of BR-responsive genes. Considered together, our results imply that HSP90 takes a part in BR-mediated gene expression through complex formation with two major transcription factors. |
| 巻・号 | 178 |
| ページ | 69-73 |
| 公開日 | 2015-4-15 |
| DOI | 10.1016/j.jplph.2015.02.003 |
| PII | S0176-1617(15)00041-3 |
| PMID | 25778412 |
| MeSH | Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Benzoquinones / pharmacology Brassinosteroids / metabolism* DNA-Binding Proteins Gene Expression Regulation / drug effects* HSP90 Heat-Shock Proteins / genetics* HSP90 Heat-Shock Proteins / metabolism Lactams, Macrocyclic / pharmacology Nuclear Proteins / genetics* Nuclear Proteins / metabolism Phosphorylation Signal Transduction Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 3.013 |
| 引用数 | 15 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |