RRC ID 37018
Author Okamoto M, Yamada L, Fujisaki Y, Bloomfield G, Yoshida K, Kuwayama H, Sawada H, Mori T, Urushihara H.
Title Two HAP2-GCS1 homologs responsible for gamete interactions in the cellular slime mold with multiple mating types: Implication for common mechanisms of sexual reproduction shared by plants and protozoa and for male-female differentiation.
Journal Dev Biol
Abstract Fertilization is a central event in sexual reproduction, and understanding its molecular mechanisms has both basic and applicative biological importance. Recent studies have uncovered the molecules that mediate this process in a variety of organisms, making it intriguing to consider conservation and evolution of the mechanisms of sexual reproduction across phyla. The social amoeba Dictyostelium discoideum undergoes sexual maturation and forms gametes under dark and humid conditions. It exhibits three mating types, type-I, -II, and -III, for the heterothallic mating system. Based on proteome analyses of the gamete membranes, we detected expression of two homologs of the plant fertilization protein HAP2-GCS1. When their coding genes were disrupted in type-I and type-II strains, sexual potency was completely lost, whereas disruption in the type-III strain did not affect mating behavior, suggesting that the latter acts as female in complex organisms. Our results demonstrate the highly conserved function of HAP2-GCS1 in gamete interactions and suggest the presence of additional allo-recognition mechanisms in D. discoideum gametes.
Volume 415(1)
Pages 6-13
Published 2016-7-1
DOI 10.1016/j.ydbio.2016.05.018
PII S0012-1606(16)30094-X
PMID 27189178
PMC PMC4910948
MeSH Amino Acid Motifs Amino Acid Sequence Arabidopsis Proteins / genetics Arabidopsis Proteins / physiology Carrier Proteins / genetics Carrier Proteins / physiology Cell Fusion Dictyostelium / genetics Dictyostelium / physiology* Fertilization Gene Knockout Techniques Genes, Protozoan* Germ Cells / physiology* Phylogeny Plant Physiological Phenomena Proteome Protozoan Proteins / biosynthesis Protozoan Proteins / genetics Protozoan Proteins / physiology* Species Specificity Transformation, Genetic
IF 2.896
Times Cited 12
Cellular slime molds S90042 S90043 S90044 S90076 S90077 S90078 S90079 S90253 S90426 S90427 S90428 S90429 S90430 S90431 S90434 S90435 S90436 S90437 S90460