RRC ID 37040
著者 Kato S, Masuda Y, Konishi M, Oikawa T.
タイトル Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography.
ジャーナル J Pharm Biomed Anal
Abstract The intrinsic D-amino acid profile of mouse macrophages extracted from the peritoneal cavity was analyzed using high performance liquid chromatography. Six D-amino acids (D-Asp, D-Ser, D-Ala, D-Leu, D-Gln and D-Lys) were detected in cell lysates of mouse macrophages. The content and the D/D+L ratio differed depending on the type of D-amino acid and were approximately 3.5-22 nmol/g cells, and approximately 1-20%, respectively. The D-amino acid composition of RAW 264.7 cells, which is a model macrophage cell line, was similar to that of the mouse macrophage. These results suggest that macrophages and RAW 264.7 cells with macrophage-like functions have a similar D-amino acid profile.
巻・号 116
ページ 101-4
公開日 2015-12-10
DOI 10.1016/j.jpba.2015.04.028
PII S0731-7085(15)00261-7
PMID 25982753
MeSH Amino Acids / analysis* Amino Acids / chemistry* Amino Acids / metabolism Animals Cell Line Chromatography, High Pressure Liquid / methods Macrophages / chemistry* Macrophages / metabolism Mice Mice, Inbred C57BL Stereoisomerism
IF 3.209
引用数 6
WOS 分野 PHARMACOLOGY & PHARMACY CHEMISTRY, ANALYTICAL
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)