Reference - Detail
| RRC ID | 37070 |
|---|---|
| Author | Kobashigawa S, Morikawa K, Mori H, Kashino G. |
| Title | Gemcitabine Induces Radiosensitization Through Inhibition of RAD51-dependent Repair for DNA Double-strand Breaks. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Gemcitabine (GEM) is used in clinical chemo-radiotherapy; however, the mechanism that contributes to enhanced radiosensitivity by GEM is not fully-understood. We evaluated the effect of GEM on radiosensitization in pancreatic cancer cell lines. MATERIALS AND METHODS:Pancreatic cell lines PK-59 and PK-45p were used. A total of 5 μM GEM for 4 h were administered pre- or post-gamma irradiation. RESULTS:Enhanced cell killing effects by GEM in radiotherapy were observed for pre-treatment but not post-treatment GEM. We focused on the dynamics of RAD51 and phospho-H2AX foci after irradiation. Significantly higher numbers of phospho-H2AX foci were observed in GEM pre-treated cells than in untreated cells after irradiation. We also found inhibition of the formation and degradation of RAD51 foci by GEM pre-treatment. The radiosensitizing effect of GEM was suppressed by knockdown of RAD51. CONCLUSION:RAD51-dependent homologous recombination is one of the key targets in the GEM-induced radiosensitizing effect. |
| Volume | 35(5) |
| Pages | 2731-7 |
| Published | 2015-5-1 |
| PII | 35/5/2731 |
| PMID | 25964552 |
| MeSH | Cell Line, Tumor DNA Breaks, Double-Stranded / drug effects DNA Repair / drug effects Deoxycytidine / administration & dosage Deoxycytidine / analogs & derivatives* Gamma Rays Gemcitabine Humans Pancreatic Neoplasms / drug therapy* Pancreatic Neoplasms / pathology Pancreatic Neoplasms / radiotherapy* Radiation Tolerance / drug effects* Radiation-Sensitizing Agents |
| IF | 1.994 |
| Times Cited | 10 |
| WOS Category | ONCOLOGY |
| Resource | |
| Human and Animal Cells | PK-59(RCB1901) PK-45P(RCB2141) |