論文 - 詳細
| RRC ID | 37070 |
|---|---|
| 著者 | Kobashigawa S, Morikawa K, Mori H, Kashino G. |
| タイトル | Gemcitabine Induces Radiosensitization Through Inhibition of RAD51-dependent Repair for DNA Double-strand Breaks. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Gemcitabine (GEM) is used in clinical chemo-radiotherapy; however, the mechanism that contributes to enhanced radiosensitivity by GEM is not fully-understood. We evaluated the effect of GEM on radiosensitization in pancreatic cancer cell lines. MATERIALS AND METHODS:Pancreatic cell lines PK-59 and PK-45p were used. A total of 5 μM GEM for 4 h were administered pre- or post-gamma irradiation. RESULTS:Enhanced cell killing effects by GEM in radiotherapy were observed for pre-treatment but not post-treatment GEM. We focused on the dynamics of RAD51 and phospho-H2AX foci after irradiation. Significantly higher numbers of phospho-H2AX foci were observed in GEM pre-treated cells than in untreated cells after irradiation. We also found inhibition of the formation and degradation of RAD51 foci by GEM pre-treatment. The radiosensitizing effect of GEM was suppressed by knockdown of RAD51. CONCLUSION:RAD51-dependent homologous recombination is one of the key targets in the GEM-induced radiosensitizing effect. |
| 巻・号 | 35(5) |
| ページ | 2731-7 |
| 公開日 | 2015-5-1 |
| PII | 35/5/2731 |
| PMID | 25964552 |
| MeSH | Cell Line, Tumor DNA Breaks, Double-Stranded / drug effects DNA Repair / drug effects Deoxycytidine / administration & dosage Deoxycytidine / analogs & derivatives* Gamma Rays Gemcitabine Humans Pancreatic Neoplasms / drug therapy* Pancreatic Neoplasms / pathology Pancreatic Neoplasms / radiotherapy* Radiation Tolerance / drug effects* Radiation-Sensitizing Agents |
| IF | 1.994 |
| 引用数 | 10 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | PK-59(RCB1901) PK-45P(RCB2141) |