論文 - 詳細
| RRC ID | 3708 |
|---|---|
| 著者 | Nakashima A, Maruki Y, Imamura Y, Kondo C, Kawamata T, Kawanishi I, Takata H, Matsuura A, Lee KS, Kikkawa U, Ohsumi Y, Yonezawa K, Kamada Y. |
| タイトル | The yeast Tor signaling pathway is involved in G2/M transition via polo-kinase. |
| ジャーナル | PLoS One |
| Abstract |
The target of rapamycin (Tor) protein plays central roles in cell growth. Rapamycin inhibits cell growth and promotes cell cycle arrest at G1 (G0). However, little is known about whether Tor is involved in other stages of the cell division cycle. Here we report that the rapamycin-sensitive Tor complex 1 (TORC1) is involved in G2/M transition in S. cerevisiae. Strains carrying a temperature-sensitive allele of KOG1 (kog1-105) encoding an essential component of TORC1, as well as yeast cell treated with rapamycin show mitotic delay with prolonged G2. Overexpression of Cdc5, the yeast polo-like kinase, rescues the growth defect of kog1-105, and in turn, Cdc5 activity is attenuated in kog1-105 cells. The TORC1-Type2A phosphatase pathway mediates nucleocytoplasmic transport of Cdc5, which is prerequisite for its proper localization and function. The C-terminal polo-box domain of Cdc5 has an inhibitory role in nuclear translocation. Taken together, our results indicate a novel function of Tor in the regulation of cell cycle and proliferation. |
| 巻・号 | 3(5) |
| ページ | e2223 |
| 公開日 | 2008-5-21 |
| DOI | 10.1371/journal.pone.0002223 |
| PMID | 18493323 |
| PMC | PMC2375053 |
| MeSH | Alleles Cell Cycle Proteins / metabolism* Cell Division* G2 Phase* Membrane Proteins / genetics Protein Kinases / metabolism* Protein Serine-Threonine Kinases / metabolism* Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Signal Transduction* |
| IF | 2.74 |
| 引用数 | 44 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BY9004 BY9943 |