RRC ID 37099
著者 Ito H, Hasegawa K, Hasegawa Y, Nishimaki T, Hosomichi K, Kimura S, Ohba M, Yao H, Onimaru M, Inoue I, Inoue H.
タイトル Silver Nanoscale Hexagonal Column Chips for Detecting Cell-free DNA and Circulating Nucleosomes in Cancer Patients.
ジャーナル Sci Rep
Abstract Blood tests, which are commonly used for cancer screening, generally have low sensitivity. Here, we developed a novel rapid and simple method to generate silver nanoscale hexagonal columns (NHCs) for use in surface-enhanced Raman scattering (SERS). We reported that the intensity of SERS spectra of clinical serum samples obtained from gastrointestinal cancer patients is was significantly higher than that of SERS spectra of clinical serum samples obtained from non-cancer patients. We estimated the combined constituents on silver NHCs by using a field emission-type scanning electron microscope, Raman microscopes, and a 3D laser scanning confocal microscope. We obtained the Raman scattering spectra of samples of physically fractured cells and clinical serum. No spectra were obtained for chemically lysed cultured cells and DNA, RNA, and protein extracted from cultured cells. We believe that our method, which uses SERS with silver NHCs to detect circulating nucleosomes bound by methylated cell-free DNA, may be successfully implemented in blood tests for cancer screening.
巻・号 5
ページ 10455
公開日 2015-5-21
DOI 10.1038/srep10455
PII srep10455
PMID 25994878
PMC PMC4440038
MeSH Cell Line, Tumor DNA / analysis* DNA / blood DNA Methylation Early Detection of Cancer Humans Microscopy, Confocal Microscopy, Electron, Scanning Nanostructures / chemistry* Neoplasms / blood Neoplasms / diagnosis* Neoplasms / pathology Nucleosomes / metabolism* Silver / chemistry* Spectrum Analysis, Raman*
IF 3.998
引用数 10
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 Kato III(RCB2088) MKN45(RCB1001) CW-2(RCB0778) PK-45P(RCB2141)