RRC ID 37116
著者 Kasajima I, Sasaki K.
タイトル A chimeric repressor of petunia PH4 R2R3-MYB family transcription factor generates margined flowers in torenia.
ジャーナル Plant Signal Behav
Abstract The development of new phenotypes is key to the commercial development of the main floricultural species and cultivars. Important new phenotypes include features such as multiple-flowers, color variations, increased flower size, new petal shapes, variegation and distinctive petal margin colourations. Although their commercial use is not yet common, the transgenic technologies provide a potentially rapid means of generating interesting new phenotypes. In this report, we construct 5 vectors which we expected to change the color of the flower anthocyanins, from purple to blue, regulating vacuolar pH. When these constructs were transformed into purple torenia, we unexpectedly recovered some genotypes having slightly margined petals. These transgenic lines expressed a chimeric repressor of the petunia PhPH4 gene under the control of Cauliflower mosaic virus 35 S RNA promoter. PhPH4 is an R2R3-type MYB transcription factor. The transgenic lines lacked pigmentation in the petal margin cells both on the adaxial and abaxial surfaces. Expressions of Flavanone 3-hydroxylase (F3H), Flavonoid 3'-hydroxylase (F3'H) and Flavonoid 3'5'-hydroxylase (F3'5'H) genes were reduced in the margins of these transgenic lines, suggesting an inhibitory effect of PhPH4 repressor on anthocyanin synthesis.
巻・号 11(5)
ページ e1177693
公開日 2016-4-19
DOI 10.1080/15592324.2016.1177693
PMID 27089475
PMC PMC4976784
MeSH Amino Acid Sequence Anthocyanins / metabolism Base Sequence DNA, Plant / isolation & purification Flowers / physiology* Gene Expression Regulation, Plant Genes, Plant Magnoliopsida / genetics Magnoliopsida / physiology* Petunia / genetics Petunia / metabolism* Phenotype Pigmentation Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified Repressor Proteins / chemistry Repressor Proteins / genetics Repressor Proteins / metabolism* Transcription Factors / chemistry Transcription Factors / genetics Transcription Factors / metabolism* Transformation, Genetic
引用数 0
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY PLANT SCIENCES
リソース情報
アサガオ DNA clones (InNHX1 InNHX2)