RRC ID |
37119
|
著者 |
Inoue H, Hayashi N, Matsushita A, Xinqiong L, Nakayama A, Sugano S, Jiang CJ, Takatsuji H.
|
タイトル |
Blast resistance of CC-NB-LRR protein Pb1 is mediated by WRKY45 through protein-protein interaction.
|
ジャーナル |
Proc Natl Acad Sci U S A
|
Abstract |
Panicle blast 1 (Pb1) is a panicle blast resistance gene derived from the indica rice cultivar "Modan." Pb1 encodes a coiled-coil-nucleotide-binding site-leucine-rich repeat (CC-NB-LRR) protein and confers durable, broad-spectrum resistance to Magnaporthe oryzae races. Here, we investigated the molecular mechanisms underlying Pb1-mediated blast resistance. The Pb1 protein interacted with WRKY45, a transcription factor involved in induced resistance via the salicylic acid signaling pathway that is regulated by the ubiquitin proteasome system. Pb1-mediated panicle blast resistance was largely compromised when WRKY45 was knocked down in a Pb1-containing rice cultivar. Leaf-blast resistance by Pb1 overexpression (Pb1-ox) was also compromised in WRKY45 knockdown/Pb1-ox rice. Blast infection induced higher accumulation of WRKY45 in Pb1-ox than in control Nipponbare rice. Overexpression of Pb1-Quad, a coiled-coil domain mutant that had weak interaction with WRKY45, resulted in significantly weaker blast resistance than that of wild-type Pb1. Overexpression of Pb1 with a nuclear export sequence failed to confer blast resistance to rice. These results suggest that the blast resistance of Pb1 depends on its interaction with WRKY45 in the nucleus. In a transient system using rice protoplasts, coexpression of Pb1 enhanced WRKY45 accumulation and increased WRKY45-dependent transactivation activity, suggesting that protection of WRKY45 from ubiquitin proteasome system degradation is possibly involved in Pb1-dependent blast resistance.
|
巻・号 |
110(23)
|
ページ |
9577-82
|
公開日 |
2013-6-4
|
DOI |
10.1073/pnas.1222155110
|
PII |
1222155110
|
PMID |
23696671
|
PMC |
PMC3677490
|
MeSH |
Cell Fractionation
Disease Resistance / genetics*
Gene Knockdown Techniques
Luciferases
Magnaporthe*
Oryza / genetics*
Oryza / microbiology
Plant Diseases / microbiology*
Plant Proteins / genetics
Plant Proteins / metabolism*
Protein Interaction Maps
Signal Transduction / genetics
Transcription Factors / metabolism*
|
IF |
9.412
|
引用数 |
101
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
rpc00031 |