| RRC ID |
37123
|
| Author |
Hattori T, Nakashima K, Marutani T, Kiso Y, Nishi Y, Mukai H.
|
| Title |
Successful acquisition of a neutralizing monoclonal antibody against a novel neutrophil-activating peptide, mitocryptide-1.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Mitocryptide-1 (MCT-1) is a novel neutrophil-activating peptide derived from mitochondrial cytochrome c oxidase subunit VIII, and its physiological role and involvement in various diseases have not yet been elucidated. Generating neutralizing antibodies against the function of MCT-1 is of particular importance for investigating its physiological and pathophysiological roles, because MCT-1 is a fragmented peptide of its mother protein and hence it is very difficult to manipulate its expression level genetically without affecting expression of the mother protein. Here, we report the successful generation of a neutralizing monoclonal antibody (MAb) against MCT-1. This MAb, designated NM1B1, which specifically bound to the region of positions 9-22 of MCT-1, showed concentration-dependent inhibition of MCT-1-induced migration and β-hexosaminidase release in neutrophilic/granulocytic differentiated HL-60 cells. Thus, NM1B1, as a neutralizing MAb against MCT-1, could elucidate not just the physiological regulatory mechanisms of MCT-1 but also its pathophysiological involvement in various inflammatory diseases in vivo.
|
| Volume |
463(1-2)
|
| Pages |
54-9
|
| Published |
2015-7-17
|
| DOI |
10.1016/j.bbrc.2015.05.016
|
| PII |
S0006-291X(15)00922-5
|
| PMID |
25986736
|
| MeSH |
Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology*
Antibodies, Neutralizing / immunology*
Antibody Specificity
Electron Transport Complex IV / chemistry
Electron Transport Complex IV / genetics
Electron Transport Complex IV / immunology*
Epitopes / chemistry
Epitopes / genetics
Female
HL-60 Cells
Humans
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Neutrophils / immunology*
Peptide Fragments / chemistry
Peptide Fragments / genetics
Peptide Fragments / immunology
Protein Subunits / chemistry
Protein Subunits / genetics
Protein Subunits / immunology
|
| IF |
2.985
|
| Times Cited |
3
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HL-60(RCB3683) |
