論文 - 詳細
| RRC ID | 37142 |
|---|---|
| 著者 | Ota S, Oshima K, Yamazaki T, Kim S, Yu Z, Yoshihara M, Takeda K, Takeshita T, Hirata A, Bišová K, Zachleder V, Hattori M, Kawano S. |
| タイトル | Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. |
| ジャーナル | Biotechnol Biofuels |
| Abstract |
BACKGROUND:Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and for biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, and starch productivity levels. However, further biotechnological exploitation has been hampered by a lack of genomic information. RESULTS:Here, we sequenced the whole genome and transcriptome, and analyzed the behavior of P. kessleri NIES-2152 under lipid production-inducing conditions. The assembly includes 13,057 protein-coding genes in a 62.5-Mbp nuclear genome. Under conditions of sulfur deprivation, lipid accumulation was correlated with the transcriptomic induction of enzymes involved in sulfur metabolism, triacylglycerol (TAG) synthesis, autophagy, and remodeling of light-harvesting complexes. CONCLUSIONS:Three-dimensional transmission electron microscopy (3D-TEM) revealed extensive alterations in cellular anatomy accompanying lipid hyperaccumulation. The present 3D-TEM results, together with transcriptomic data support the finding that upregulation of TAG synthesis and autophagy are potential key mediators of the hyperaccumulation of lipids under conditions of nutrient stress. |
| 巻・号 | 9 |
| ページ | 13 |
| 公開日 | 2016-1-1 |
| DOI | 10.1186/s13068-016-0424-2 |
| PII | 424 |
| PMID | 26811738 |
| PMC | PMC4724957 |
| IF | 4.815 |
| 引用数 | 35 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENERGY & FUELS |
| リソース情報 | |
| 藻類 | NIES-2152 |