RRC ID 37462
Author Ota S, Yoshihara M, Yamazaki T, Takeshita T, Hirata A, Konomi M, Oshima K, Hattori M, Bišová K, Zachleder V, Kawano S.
Title Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri.
Journal Sci Rep
Abstract Phosphorus is an essential element for life on earth and is also important for modern agriculture, which is dependent on inorganic fertilizers from phosphate rock. Polyphosphate is a biological polymer of phosphate residues, which is accumulated in organisms during the biological wastewater treatment process to enhance biological phosphorus removal. Here, we investigated the relationship between polyphosphate accumulation and electron-dense bodies in the green alga Parachlorella kessleri. Under sulfur-depleted conditions, in which some symporter genes were upregulated, while others were downregulated, total phosphate accumulation increased in the early stage of culture compared to that under sulfur-replete conditions. The P signal was detected only in dense bodies by energy dispersive X-ray analysis. Transmission electron microscopy revealed marked ultrastructural variations in dense bodies with and without polyphosphate. Our findings suggest that the dense body is a site of polyphosphate accumulation, and P. kessleri has potential as a phosphate-accumulating organism.
Volume 6
Pages 25731
Published 2016-5-16
DOI 10.1038/srep25731
PII srep25731
PMID 27180903
PMC PMC4867602
MeSH Chlorophyta / cytology Chlorophyta / growth & development Chlorophyta / metabolism* Chlorophyta / ultrastructure Electrons* Imaging, Three-Dimensional Lipids / chemistry* Models, Biological Phosphates / metabolism* Polyphosphates / metabolism Sequence Analysis, RNA Staining and Labeling Sulfur / metabolism Transcriptome / genetics
IF 3.998
Times Cited 17
Algae NIES-2152