| RRC ID |
37464
|
| Author |
Sun LW, Jiang WJ, Sato H, Kawachi M, Lu XW.
|
| Title |
Rapid Classification and Identification of Microcystis aeruginosa Strains Using MALDI-TOF MS and Polygenetic Analysis.
|
| Journal |
PLoS One
|
| Abstract |
Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was used to establish a rapid, simple, and accurate method to differentiate among strains of Microcystis aeruginosa, one of the most prevalent types of bloom-forming cyanobacteria. M. aeruginosa NIES-843, for which a complete genome has been sequenced, was used to characterize ribosomal proteins as biomarkers and to optimize conditions for observing ribosomal proteins as major peaks in a given mass spectrum. Thirty-one of 52 ribosomal subunit proteins were detected and identified along the mass spectrum. Fifty-five strains of M. aeruginosa from different habitats were analyzed using MALDI-TOF MS; among these samples, different ribosomal protein types were observed. A polygenetic analysis was performed using an unweighted pair-group method with arithmetic means and different ribosomal protein types to classify the strains into five major clades. Two clades primarily contained toxic strains, and the other three clades contained exclusively non-toxic strains. This is the first study to differentiate cyanobacterial strains using MALDI-TOF MS.
|
| Volume |
11(5)
|
| Pages |
e0156275
|
| Published |
2016-1-1
|
| DOI |
10.1371/journal.pone.0156275
|
| PII |
PONE-D-16-08617
|
| PMID |
27227555
|
| PMC |
PMC4881969
|
| MeSH |
Microcystis / classification
Microcystis / genetics*
Microcystis / metabolism*
Models, Genetic*
Phylogeny
Ribosomal Proteins / genetics*
Ribosomal Proteins / metabolism*
Ribosome Subunits / genetics
Ribosome Subunits / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
| IF |
2.74
|
| Times Cited |
5
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Algae |
NIES-44
NIES-87
NIES-88
NIES-89
NIES-90
NIES-100
NIES-101
NIES-104
NIES-105
NIES-107
NIES-109
NIES-111
NIES-112
NIES-478
NIES-604
NIES-843
NIES-901
NIES-1043
NIES-1053
NIES-1058
NIES-1061
NIES-1068
NIES-1069
NIES-1070
NIES-1072
NIES-1076
NIES-1077
NIES-1085
NIES-1094
NIES-1099
NIES-1101
NIES-1104
NIES-1105
NIES-1113
NIES-1115
NIES-1126
NIES-1134
NIES-1135
NIES-1144
NIES-1151
NIES-1157
NIES-1170
NIES-1211
NIES-1213
NIES-1215
NIES-1216
NIES-1217
NIES-1218
NIES-1222
NIES-1224
NIES-1230
NIES-1232
NIES-1233
NIES-1239 |
