論文 - 詳細
| RRC ID | 37470 |
|---|---|
| 著者 | Yamagishi T, Kurihara A, Kawai H. |
| タイトル | A Ribbon-like Structure in the Ejective Organelle of the Green Microalga Pyramimonas parkeae (Prasinophyceae) Consists of Core Histones and Polymers Containing N-acetyl-glucosamine. |
| ジャーナル | Protist |
| Abstract |
The green microalga, Pyramimonas parkeae (Prasinophyceae) has an ejective organelle containing a coiled ribbon structure resembling the ejectisome in Cryptophyta. This structure is discharged from the cell by a stimulus and extends to form a tube-like structure, but the molecular components of the structure have not been identified. Tricine-SDS-PAGE analysis indicated that the ribbon-like structure of P. parkeae contains some proteins and low molecular acidic polymers. Edman degradation, LC/MS/MS analyses and immunological studies demonstrated that their proteins are core histones (H3, H2A, H2B and H4). In addition, monosaccharide composition analysis of the ribbon-like structures and degradation by lysozyme strongly indicated that the ribbon-like structure consist of β (1-4) linked polymers containing N-acetyl-glucosamine. Purified polymers and recombinant histones formed glob-like or filamentous structures. Therefore we conclude that the ribbon-like structure of P. parkeae mainly consists of a complex of core histones (H3, H2A, H2B and H4) and polymers containing N-acetyl-glucosamine, and suggest to name the ejective organelle in P. parkeae the "histrosome" to distinguish it from the ejectisome in Cryptophyta. |
| 巻・号 | 166(5) |
| ページ | 522-33 |
| 公開日 | 2015-11-1 |
| DOI | 10.1016/j.protis.2015.08.003 |
| PII | S1434-4610(15)00051-6 |
| PMID | 26398336 |
| MeSH | Acetylglucosamine / metabolism* Algal Proteins / chemistry Algal Proteins / genetics* Algal Proteins / metabolism Chlorophyta / genetics* Chlorophyta / metabolism Histones / chemistry Histones / genetics* Histones / metabolism Molecular Sequence Data Organelles / metabolism Phylogeny Polymers / metabolism Sequence Analysis, DNA |
| IF | 1.989 |
| 引用数 | 3 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-251 NIES-254 |