RRC ID 37470
Author Yamagishi T, Kurihara A, Kawai H.
Title A Ribbon-like Structure in the Ejective Organelle of the Green Microalga Pyramimonas parkeae (Prasinophyceae) Consists of Core Histones and Polymers Containing N-acetyl-glucosamine.
Journal Protist
Abstract The green microalga, Pyramimonas parkeae (Prasinophyceae) has an ejective organelle containing a coiled ribbon structure resembling the ejectisome in Cryptophyta. This structure is discharged from the cell by a stimulus and extends to form a tube-like structure, but the molecular components of the structure have not been identified. Tricine-SDS-PAGE analysis indicated that the ribbon-like structure of P. parkeae contains some proteins and low molecular acidic polymers. Edman degradation, LC/MS/MS analyses and immunological studies demonstrated that their proteins are core histones (H3, H2A, H2B and H4). In addition, monosaccharide composition analysis of the ribbon-like structures and degradation by lysozyme strongly indicated that the ribbon-like structure consist of β (1-4) linked polymers containing N-acetyl-glucosamine. Purified polymers and recombinant histones formed glob-like or filamentous structures. Therefore we conclude that the ribbon-like structure of P. parkeae mainly consists of a complex of core histones (H3, H2A, H2B and H4) and polymers containing N-acetyl-glucosamine, and suggest to name the ejective organelle in P. parkeae the "histrosome" to distinguish it from the ejectisome in Cryptophyta.
Volume 166(5)
Pages 522-33
Published 2015-11-1
DOI 10.1016/j.protis.2015.08.003
PII S1434-4610(15)00051-6
PMID 26398336
MeSH Acetylglucosamine / metabolism* Algal Proteins / chemistry Algal Proteins / genetics* Algal Proteins / metabolism Chlorophyta / genetics* Chlorophyta / metabolism Histones / chemistry Histones / genetics* Histones / metabolism Molecular Sequence Data Organelles / metabolism Phylogeny Polymers / metabolism Sequence Analysis, DNA
IF 1.989
Times Cited 3
Algae NIES-251 NIES-254