論文 - 詳細
RRC ID | 37522 |
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著者 | Saitoh Y, Yoshimoto T, Kato S, Miwa N. |
タイトル | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell. |
ジャーナル | Exp Oncol |
Abstract |
AIM:In this study, we evaluated the carcinostatic effects of combined ascorbic acid (AsA) and a capacitive-resistive electric transfer (CRet) hyperthermic apparatus-induced hyperthermic treatment on Ehrlich ascites tumor (EAT) cells. MATERIALS AND METHODS:EAT cells were exposed to various AsA (0-10 mM) concentrations for 1 h; they subsequently underwent CRet treatment for 15 min at 42 °C. Cell viability was assessed by the WST-8 assay 24 h after the combined treatment. Reactive oxygen species involvement was evaluated using catalase and tempol; caspase-3/7 activation was determined by their fluorescent substrates; cell proliferation were estimated by time-lapse observation. The effect on the cell cycle was analyzed by flow cytometry. RESULTS:Combined AsA and CRet treatment synergistically suppressed cell viability compared with either treatment alone, and these synergistically carcinostatic effects were evident even at noncytotoxic concentrations of AsA alone (≤ 2 mM). The carcinostatic effects of combined AsA and CRet treatment were attenuated in a dose-dependent manner by catalase addition, but not by the superoxide anion radical scavenger tempol. Time-lapse observation revealed that combined AsA and CRet treatment activated caspase-3/7 at 10-24 h after treatment, accompanied by significant cell growth suppression. Cell cycle analysis revealed that the rate of sub-G1-phase (apoptotic) cells was drastically increased at 12 h and 24 h, and that the G2/M-phase cells gradually increased at 6-24 h after treatment. CONCLUSION:These results indicate that combined AsA and CRet treatment synergistically inhibits EAT cell growth through G2/M arrest and apoptosis induction via H2O2 generation at lower AsA concentrations; this carcinostatic effect cannot be exerted by AsA alone. |
巻・号 | 37(2) |
ページ | 94-9 |
公開日 | 2015-6-1 |
PII | 7720 |
PMID | 26112934 |
MeSH | Animals Antineoplastic Agents / pharmacology* Apoptosis Ascorbic Acid / pharmacology* Carcinoma, Ehrlich Tumor / therapy* Cell Cycle Checkpoints Cell Line, Tumor Cell Proliferation Cell Survival Combined Modality Therapy Drug Screening Assays, Antitumor Hyperthermia, Induced Reactive Oxygen Species / metabolism |
リソース情報 | |
ヒト・動物細胞 | Ehrlich(RCB0142) |