RRC ID 37556
著者 Sakaguchi M, Watanabe M, Kinoshita R, Kaku H, Ueki H, Futami J, Murata H, Inoue Y, Li SA, Huang P, Putranto EW, Ruma IM, Nasu Y, Kumon H, Huh NH.
タイトル Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene.
ジャーナル Mol Biotechnol
Abstract For expression of genes in mammalian cells, various vectors have been developed using promoters including CMV, EF-1α, and CAG promoters and have been widely used. However, such expression vectors sometimes fail to attain sufficient expression levels depending on the nature of cargo genes and/or on host cell types. In the present study, we aimed to develop a potent promoter system that enables high expression levels of cargo genes ubiquitously in many different cell types. We found that insertion of an additional promoter downstream of a cargo gene greatly enhanced the expression levels. Among the constructs we tested, C-TSC cassette (C: CMV-RU5' located upstream; TSC: another promoter unit composed of triple tandem promoters, hTERT, SV40, and CMV, located downstream of the cDNA plus a polyadenylation signal) had the most potent capability, showing far higher efficiency than that of potent conventional vector systems. The results indicate that the new expression system is useful for production of recombinant proteins in mammalian cells and for application as a gene therapeutic measure.
巻・号 56(7)
ページ 621-30
公開日 2014-7-1
DOI 10.1007/s12033-014-9738-0
PMID 24526517
PMC PMC4067539
MeSH Animals Gene Expression Regulation Genetic Vectors* Humans Promoter Regions, Genetic* Telomerase / biosynthesis* Telomerase / genetics Transgenes
IF 2.022
引用数 33
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 pIDT-SMART(C-TSC) (RDB14136)